Protein Info for ECOLIN_RS04100 in Escherichia coli Nissle 1917

Annotation: anion permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 477 transmembrane" amino acids 7 to 25 (19 residues), see Phobius details amino acids 31 to 48 (18 residues), see Phobius details amino acids 55 to 73 (19 residues), see Phobius details amino acids 86 to 107 (22 residues), see Phobius details amino acids 115 to 139 (25 residues), see Phobius details amino acids 147 to 166 (20 residues), see Phobius details amino acids 178 to 204 (27 residues), see Phobius details amino acids 220 to 243 (24 residues), see Phobius details amino acids 273 to 291 (19 residues), see Phobius details amino acids 297 to 315 (19 residues), see Phobius details amino acids 326 to 345 (20 residues), see Phobius details amino acids 363 to 381 (19 residues), see Phobius details amino acids 388 to 415 (28 residues), see Phobius details amino acids 422 to 438 (17 residues), see Phobius details amino acids 445 to 468 (24 residues), see Phobius details PF00939: Na_sulph_symp" amino acids 4 to 474 (471 residues), 647.9 bits, see alignment E=1.2e-198 TIGR00785: transporter, divalent anion:Na+ symporter (DASS) family" amino acids 23 to 468 (446 residues), 553.3 bits, see alignment E=1.8e-170 PF03600: CitMHS" amino acids 44 to 410 (367 residues), 61.7 bits, see alignment E=6.8e-21 amino acids 285 to 459 (175 residues), 37.2 bits, see alignment E=2e-13

Best Hits

Swiss-Prot: 99% identical to YBHI_ECOLI: Inner membrane protein YbhI (ybhI) from Escherichia coli (strain K12)

KEGG orthology group: K03319, divalent anion:Na+ symporter, DASS family (inferred from 100% identity to ecq:ECED1_0733)

Predicted SEED Role

"2-oxoglutarate/malate translocator" in subsystem Photorespiration (oxidative C2 cycle)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (477 amino acids)

>ECOLIN_RS04100 anion permease (Escherichia coli Nissle 1917)
MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIVGLVIKPFPEPVVLLIA
VAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAAFVTTGLGKRIAYLLIGKI
GSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSVAVALGSEPEKSPRRVGHYLM
MSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISWGGWALAAGLPGIIMLLVTPLVI
YTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLGVFVLALLGWIFSKSLGVDESTVAI
VVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGIIGLSSLLSKVKFFEWLAEVFKNNLAFD
GHGNVAFFVIIFLSIIVRYFFASGSAYIVAMLPVFAMLANVSGAPLMLTALALLFSNSYG
GMVTHYGGAAGPVIFGVGYNDIKSWWLVGAVLTILTFLVHITLGVWWWNMLIGWNML