Protein Info for ECOLIN_RS03350 in Escherichia coli Nissle 1917
Annotation: triphosphoribosyl-dephospho-CoA synthase CitG
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to CITG_ECO81: 2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (citG) from Escherichia coli O81 (strain ED1a)
KEGG orthology group: K05966, triphosphoribosyl-dephospho-CoA synthase [EC: 2.7.8.25] (inferred from 97% identity to eco:b0613)MetaCyc: 97% identical to triphosphoribosyl-dephospho-CoA synthase (Escherichia coli K-12 substr. MG1655)
2.7.8.25-RXN [EC: 2.4.2.52]
Predicted SEED Role
"2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme-A synthase (EC 2.7.8.25)" (EC 2.7.8.25)
MetaCyc Pathways
- malonate decarboxylase activation (1/2 steps found)
- citrate lyase activation (3/5 steps found)
Isozymes
No predicted isozymesUse Curated BLAST to search for 2.4.2.52 or 2.7.8.25
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (292 amino acids)
>ECOLIN_RS03350 triphosphoribosyl-dephospho-CoA synthase CitG (Escherichia coli Nissle 1917) MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDRINCGAHKDMALEDFHR SALAIQGWLPRFIEFGACSAEMAPEAVLNGLRPIGMACEGDMFRATAGVNTHKGSIFSLG LLCAAIGRLLQLNQSVTPITICATAASFCRGLTDRELRTNNSQLTAGQRLYQQLGLTGAR GEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLLLMATNGDTNVASRGGEGGLRWL QREAQTLLNNGGIRTPADLDYLRQFDRECIERNISPGGSADLLILTWFLAQI