Protein Info for ECOLIN_RS03345 in Escherichia coli Nissle 1917

Annotation: citrate/succinate antiporter CitT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 487 transmembrane" amino acids 7 to 27 (21 residues), see Phobius details amino acids 33 to 50 (18 residues), see Phobius details amino acids 57 to 75 (19 residues), see Phobius details amino acids 95 to 117 (23 residues), see Phobius details amino acids 138 to 156 (19 residues), see Phobius details amino acids 163 to 178 (16 residues), see Phobius details amino acids 192 to 214 (23 residues), see Phobius details amino acids 233 to 258 (26 residues), see Phobius details amino acids 287 to 304 (18 residues), see Phobius details amino acids 310 to 329 (20 residues), see Phobius details amino acids 339 to 360 (22 residues), see Phobius details amino acids 380 to 412 (33 residues), see Phobius details amino acids 424 to 444 (21 residues), see Phobius details amino acids 463 to 485 (23 residues), see Phobius details PF00939: Na_sulph_symp" amino acids 6 to 486 (481 residues), 649.7 bits, see alignment E=3.3e-199 TIGR00785: transporter, divalent anion:Na+ symporter (DASS) family" amino acids 25 to 480 (456 residues), 573.4 bits, see alignment E=1.5e-176 PF03600: CitMHS" amino acids 46 to 414 (369 residues), 70.4 bits, see alignment E=1.6e-23 amino acids 306 to 471 (166 residues), 40.9 bits, see alignment E=1.5e-14

Best Hits

Swiss-Prot: 100% identical to CITT_ECO57: Citrate/succinate antiporter (citT) from Escherichia coli O157:H7

KEGG orthology group: K09477, citrate:succinate antiporter (inferred from 100% identity to eco:b0612)

MetaCyc: 100% identical to citrate:succinate antiporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-201

Predicted SEED Role

"Citrate Succinate antiporter (TC 2.A.47.3.2)" (TC 2.A.47.3.2)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (487 amino acids)

>ECOLIN_RS03345 citrate/succinate antiporter CitT (Escherichia coli Nissle 1917)
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
IAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSSTTVWLVFGAFIFALGYEVS
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
IDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN