Protein Info for ECOLIN_RS03150 in Escherichia coli Nissle 1917

Annotation: phenylalanine transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 460 transmembrane" amino acids 28 to 48 (21 residues), see Phobius details amino acids 54 to 71 (18 residues), see Phobius details amino acids 107 to 130 (24 residues), see Phobius details amino acids 136 to 154 (19 residues), see Phobius details amino acids 163 to 184 (22 residues), see Phobius details amino acids 205 to 228 (24 residues), see Phobius details amino acids 249 to 271 (23 residues), see Phobius details amino acids 291 to 309 (19 residues), see Phobius details amino acids 340 to 361 (22 residues), see Phobius details amino acids 367 to 388 (22 residues), see Phobius details amino acids 408 to 428 (21 residues), see Phobius details amino acids 434 to 453 (20 residues), see Phobius details PF00324: AA_permease" amino acids 27 to 454 (428 residues), 431.2 bits, see alignment E=4.8e-133 PF13520: AA_permease_2" amino acids 31 to 428 (398 residues), 132.3 bits, see alignment E=2.5e-42

Best Hits

Swiss-Prot: 100% identical to PHEP_ECOLI: Phenylalanine-specific permease (pheP) from Escherichia coli (strain K12)

KEGG orthology group: K11732, phenylalanine-specific permease (inferred from 100% identity to eco:b0576)

MetaCyc: 100% identical to phenylalanine:H+ symporter PheP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-56

Predicted SEED Role

"Phenylalanine-specific permease" in subsystem Aromatic amino acid degradation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (460 amino acids)

>ECOLIN_RS03150 phenylalanine transporter (Escherichia coli Nissle 1917)
MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLGIGPAIQMAGPAVLLGY
GVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFLSGWNYWVMFVLVGMAELT
AAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGETEFWFALIKVLAIIGMIGFGL
WLLFSGHGGEKASINNLWRYGGFFATGWNGLILSLAVIMFSFGGLELIGITAAEARDPEK
SIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSNSSPFVMIFHNLDSNVVASALNFVI
LVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTRVSRRGVPINSLMLSGAITSLVVLINY
LLPQKAFGLLMALVVATLLLNWIMICLAHLRFRAAMRRQGRETQFKALLYPFGNYLCIAF
LGMILLLMCTMDDMRLSAILLPVWIVFLFVAFKTLRRKVN