Protein Info for ECOLIN_RS01070 in Escherichia coli Nissle 1917
Annotation: proline--tRNA ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to SYP_ECO81: Proline--tRNA ligase (proS) from Escherichia coli O81 (strain ED1a)
KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to ecg:E2348C_0200)MetaCyc: 98% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]
Predicted SEED Role
"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)
MetaCyc Pathways
- tRNA charging (20/21 steps found)
- colibactin biosynthesis (24/38 steps found)
- anguibactin biosynthesis (2/8 steps found)
- pyoluteorin biosynthesis (1/7 steps found)
- thiocoraline biosynthesis (1/7 steps found)
- ergotamine biosynthesis (4/12 steps found)
- (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
- pyochelin biosynthesis (2/11 steps found)
- clorobiocin biosynthesis (1/10 steps found)
- superpathway of ergotamine biosynthesis (5/18 steps found)
- pyrrolomycin biosynthesis (1/14 steps found)
- sulfazecin biosynthesis (1/16 steps found)
- phosalacine biosynthesis (2/25 steps found)
- phosphinothricin tripeptide biosynthesis (2/25 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.1.1.16
Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (572 amino acids)
>ECOLIN_RS01070 proline--tRNA ligase (Escherichia coli Nissle 1917) MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLPTGVRVLKKVENIVREE MNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDRPFVLGPTHEEVITDLIRN ELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDAYSFHTSQESLQETYDAMYAA YSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGEDDVVFSDTSDYAANIELAEAIA PKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEKTVKTLLVKAVEGSSFPLVALLVRG DHELNEVKAEKLPQVASPLTFATEEEIRAVVKAGPGSLGPVNMPIPVVIDRTVAAMSDFA AGANIDGKHYFGINWDRDVATPEIADIRNVVAGDPSPDGQGTLLIKRGIEVGHIFQLGTK YSEALKASVQGEDGRNQILTMGCYGIGVTRVVAAAIEQNYDERGIVWPDAIAPFQVAILP MNMHKSFRVQELAEKLYSELRAQGIEVLLDDRKERPGVMFADMELIGIPHTIVLGDRNLD NDDIEYKYRRNGEKQLIKTGDIVDYLVKQIKG