Protein Info for Dsui_3141 in Dechlorosoma suillum PS

Annotation: histidyl-tRNA synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 432 TIGR00442: histidine--tRNA ligase" amino acids 7 to 404 (398 residues), 515 bits, see alignment E=7.2e-159 PF13393: tRNA-synt_His" amino acids 11 to 313 (303 residues), 165.1 bits, see alignment E=4.7e-52 PF00587: tRNA-synt_2b" amino acids 69 to 320 (252 residues), 81.5 bits, see alignment E=1.6e-26 PF03129: HGTP_anticodon" amino acids 330 to 422 (93 residues), 50.8 bits, see alignment E=3.2e-17

Best Hits

Swiss-Prot: 79% identical to SYH_DECAR: Histidine--tRNA ligase (hisS) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K01892, histidyl-tRNA synthetase [EC: 6.1.1.21] (inferred from 78% identity to app:CAP2UW1_2112)

MetaCyc: 57% identical to histidine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Histidine--tRNA ligase. [EC: 6.1.1.21]

Predicted SEED Role

"Histidyl-tRNA synthetase (EC 6.1.1.21)" (EC 6.1.1.21)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.21

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QI70 at UniProt or InterPro

Protein Sequence (432 amino acids)

>Dsui_3141 histidyl-tRNA synthetase (Dechlorosoma suillum PS)
MSQKTLQAVRGMNDILPDEAELWERFEEVVRSWLHSYGYRPIRMPVVEPTGLFKRAIGEV
TDIVEKEMYSFEDALNGENLTLRPEGTAGCVRAVIQHSLAAQHPRRLYYMGQMFRHERPQ
RGRYRQFHQVGVESFGFAGPDVDAEQILMGARLWDDLGLEGITLQINSLGQPEERARHRA
ELIAYFQANADRLDEDSQRRLHTNPLRILDTKNPDMQELCANAPKLIDFLGAESLAHFEG
VQQVLRDAGQPYTVNPRLVRGLDYYNLTVFEWVTDRLGAQGTVCAGGRYDGLVEQLGGKP
TPACGFAMGVERLIALIQDQDLAGNPEPVDVYVVHQGEAASRLAFRVAEGLRDNGLAVLL
NCGGGSFKSQMKKADGSGAAFAVIIGDDEAAAGEVSLKSLRSADGEKGEQQRVKVDDLPA
TLMDSIIDWEEE