Protein Info for Dsui_3018 in Dechlorosoma suillum PS

Annotation: OAH/OAS sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 9 to 425 (417 residues), 597.6 bits, E=5.6e-184
• PF01053: Cys_Met_Meta_PP
  • amino acids 10 to 425 (416 residues), 445.3 bits, E=2.1e-137
• PF01041: DegT_DnrJ_EryC1
  • amino acids 63 to 186 (124 residues), 24.1 bits, E=3.1e-09

Best Hits

Swiss-Prot: 50% identical to FUB7_FUSO4: Sulfhydrylase FUB7 (FUB7) from Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935 / NRRL 34936)

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 78% identity to cti:RALTA_A1225)

MetaCyc: 48% identical to O-acetylhomoserine sulfhydrylase (Corynebacterium glutamicum ATCC 13032)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• homocysteine and cysteine interconversion (3/4 steps found)
• L-methionine biosynthesis II (4/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (3/5 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• L-methionine biosynthesis I (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See G8QHG0 at UniProt or InterPro

Protein Sequence (433 amino acids)

>Dsui_3018 OAH/OAS sulfhydrylase (Dechlorosoma suillum PS)
MAGKDGYTLDTLALHAGQVPDPQYGARATPIYLSTSFVFKDSDQAAALFNMERAGHVYSR
ISNPTVSVLEERIAALEGGVGAIATASGQAAMHLAVATLMGAGSHIVASGALYGGSHNLL
DYTLRRFGIETTFVPTRDVDAWRAAIRPNTRLLFGETLGNPGLDVLDIPRIAALAHEHGL
PLLVDSTFTSPYLLKPFEHGADLVFHSATKFLCGHGTAVGGVLVDSGNFDWEASGKFPTL
TEPYDGFHGMDFAEESTVAAFLLRARREGLRDFGACMSPMNAFQILQGVETLPLRMQRHV
ENARKLVDFLAAHEAVESVAYPGLESHPDHALAQKLLPRGCGSVFSFSVKGGRPAGKKFI
ETLKLFSHLANVGDAKSLVIHPASTTHFRMSDEALLAAGIGAGTIRLSVGLEDADDLIED
LSRGLAAAAKAAA