Protein Info for Dsui_2868 in Dechlorosoma suillum PS

Annotation: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 655 transmembrane" amino acids 75 to 95 (21 residues), see Phobius details PF05430: Methyltransf_30" amino acids 128 to 249 (122 residues), 121.1 bits, see alignment E=7e-39 PF01266: DAO" amino acids 264 to 619 (356 residues), 170.2 bits, see alignment E=2.4e-53 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 265 to 646 (382 residues), 371 bits, see alignment E=4e-115 PF00890: FAD_binding_2" amino acids 265 to 296 (32 residues), 22.4 bits, see alignment (E = 1.7e-08) PF13450: NAD_binding_8" amino acids 267 to 305 (39 residues), 33.6 bits, see alignment 9.5e-12

Best Hits

Swiss-Prot: 58% identical to MNMC_AROAE: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (mnmC) from Aromatoleum aromaticum (strain EbN1)

KEGG orthology group: None (inferred from 58% identity to eba:ebA6842)

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) / FAD-dependent cmnm(5)s(2)U34 oxidoreductase" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QFU1 at UniProt or InterPro

Protein Sequence (655 amino acids)

>Dsui_2868 tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain protein (Dechlorosoma suillum PS)
MSTPASPAPHSVADANPDAYRPLVPARLCRNDAGVPYSPDYDDIYHTAAGALGQARHVFL
AGNGLPRRWQQRESFTIVETGFGIGLNFLATWAAWRDDPAACRRLHFVSLEKHPFSAADL
GELHAAHPELAELAAELRAQWPLLTPGVHRLHFAGGRVSLTLFFGDAQSLLPQLRLAADA
FYLDGFAPAKNPDLWSPFVFRGLARCAAAGATLATWSVAAGVREGLQAVGFVSDKAPGFG
GKRDMLQGQYRGPGVRRTVMPGKRILVIGAGFAGTSTAHRLAERGYQVTLLDQAEGPGCG
ASGNRVGVLRPLPSLDDNRLSRLTRSAFLYTRRHLLALSAAGLPVRWGASGVLHLARDKT
HEATQRQVAETFAAPELFRYVDATESSRLIGWPVSVGGWHFPGGGWGMPPSLCQANLQAH
PAIEQRYGQRVEKLEAGADGWAAYAADGTLLAEADQVVLANAADARRLGGCDWLPLRSAR
GQATLVPETATPPLDMVVCRLGYVTPPLDGLRVCGASFLAGDEDPLPRLEEQQDNLAKLN
FILPGFAKDLAAADLGSRVGFRPISPDRLPMLGPLPENTTPATGARLQTLPRRPGLWLAS
GFGARGFVWSAWAGEFLASQIAGDPLPLEGDLADALDPARFLLKPVKAARFNDRE