Protein Info for Dsui_2718 in Dechlorosoma suillum PS

Annotation: polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 707 TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 11 to 692 (682 residues), 1091.2 bits, see alignment E=0 PF01138: RNase_PH" amino acids 15 to 144 (130 residues), 102.2 bits, see alignment E=7.7e-33 amino acids 324 to 456 (133 residues), 96.8 bits, see alignment E=3.4e-31 PF03725: RNase_PH_C" amino acids 147 to 210 (64 residues), 52.4 bits, see alignment E=1e-17 PF03726: PNPase" amino acids 243 to 320 (78 residues), 68.8 bits, see alignment E=1.2e-22 PF00013: KH_1" amino acids 556 to 613 (58 residues), 47.3 bits, see alignment 3.5e-16 PF00575: S1" amino acids 619 to 690 (72 residues), 58.2 bits, see alignment E=2.2e-19

Best Hits

Swiss-Prot: 83% identical to PNP_DECAR: Polyribonucleotide nucleotidyltransferase (pnp) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 83% identity to dar:Daro_2448)

MetaCyc: 67% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QPG4 at UniProt or InterPro

Protein Sequence (707 amino acids)

>Dsui_2718 polyribonucleotide nucleotidyltransferase (Dechlorosoma suillum PS)
MFNIAKKTFTYGAHQVTLETGEIARQASGAVLVTVDDTVVLVTVVGAKQAKPGQDFFPLT
VDYVEKTYAAGKIPGGFFKREGRPSEKETLTSRLIDRPIRPLFPEGFYNEVQVVATVMSL
NPEVDSDIPALIGASAALAISGIPFSGPIGAARVGYIDGQYVLNPSATQLKDSKLDLVVA
GTQSAVLMVESEAKELSEEVMLGAVVYGHEQMQAVINAINELVEEAGKPEWEWQAPAQDQ
VLVGKLEALVKAKLEEAYSITSKQTRSQRVKEIRSEAVAALCQGEDAPDENTVADLFFAM
EAAIVRGRILAGEPRIDGRDTRTVRPITVRTGVLPRTHGSALFTRGETQGLVVATLGTSR
DEQIIDALAGEYKDRFMLHYNMPPYATGETGRVGSPKRREIGHGRLAKRALLAVLPPADE
FSYSMRVVSEITESNGSSSMASVCGGSLALMDAGVPLKSHVAGIAMGLIKEGNRFAVLTD
ILGDEDHLGDMDFKVAGTDSGITALQMDIKIQGITKEIMAVALDQAKAGRLHILNIMQDA
QDAPRTEMSAYAPRLYTMKINPEKIRDVIGKGGAVIRALTEETGTSIDIQDDGTITIAST
DGEAAEMARKRIEAITAEVEVGKVYEGPVLKILDFGAIVSVLPGKDGLLHISQIANERVN
AVADYLKEGQVVRVKVLETDEKGRIRLSMKAVAAEEAAPAVAAEPQQ