Protein Info for Dsui_2677 in Dechlorosoma suillum PS

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 5 to 785 (781 residues), 1190 bits, E=0
• PF01326: PPDK_N
  • amino acids 17 to 345 (329 residues), 416.3 bits, E=1.5e-128
• PF00391: PEP-utilizers
  • amino acids 385 to 455 (71 residues), 99.9 bits, E=7.4e-33
• PF02896: PEP-utilizers_C
  • amino acids 479 to 781 (303 residues), 213.6 bits, E=5.4e-67

Best Hits

Swiss-Prot: 71% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 78% identity to azo:azo2167)

MetaCyc: 65% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (22/26 steps found)
• superpathway of anaerobic sucrose degradation (16/19 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• gluconeogenesis I (11/13 steps found)
• glycolysis I (from glucose 6-phosphate) (11/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (14/19 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• superpathway of hexitol degradation (bacteria) (12/18 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (11/17 steps found)
• superpathway of N-acetylneuraminate degradation (13/22 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (8/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Sequence Analysis Tools

See G8QPC6 at UniProt or InterPro

Protein Sequence (791 amino acids)

>Dsui_2677 phosphoenolpyruvate synthase (Dechlorosoma suillum PS)
MSDYVIGFDQLRMTDVDRVGGKNASLGEMISQLANTGVRVPGGFATTAHAYREFLAHEGL
AARIEAELSNLDVDNVNALLAAGAKIRQWIIDTPFPAQLQNEIKAHYDALIADSTADASF
AVRSSATAEDLPDASFAGQQETFLNIHGFDNILHAMKEVFASLYNDRAISYRVHKGFTHA
EVALSAGVQRMVRSDKGAAGVMFTLDTESGFRDAVFVTASYGLGETVVQGAVNPDEFYVH
KPMLAAGKKAVIRRNLGSKLIKMTFSAEKVAGKSTITEDVAEAERHRFAINDAEVEELAR
YAMIIEKHYQRPMDIEWGKDGVDGKLYILQARPETVKSQEGHGRVEKFKLKSFSKVLASG
RAIGQKIGVGPVRVVHDPSEMDQVKPGDVLVADMTDPNWEPVMKRASAIVTNRGGRTCHA
AIIARELGIPAIVGCGDATETLAEGEVVTVSCTEGDTGHVYRGKLDFEVTSRDLGAMPEI
KTKIMMNVGNPELAFEFSQLPSAGVGLARVEFVINNVIGIHPKAILEIDRLPASKREEIL
RRARGYANPVEFFVEKLAEGVSTIAAAFWPKPVIVRLSDFKSNEYRKLLGGDLYEPEEEN
PMLGFRGASRYIASTFRDCFEMECRAMKKVREEMGLTNVELMVPFVRTVDEGKGVVSLLA
EHGLKQGENNLKLIMMCEIPSNALLADQFLEVFDGFSIGSNDLTQLTLGLDRDSGLVASL
FDERDPAVKTLLAMAIASANKKGKYVGICGQGPSDHPDLAEWLMDQGISSISLNPDTVVD
TWTRLGAHKAG