Protein Info for Dsui_2538 in Dechlorosoma suillum PS
Annotation: acetoacetyl-CoA reductase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 74% identical to NODG_AZOBR: Nodulation protein G (nodG) from Azospirillum brasilense
KEGG orthology group: K00023, acetoacetyl-CoA reductase [EC: 1.1.1.36] (inferred from 75% identity to app:CAP2UW1_3919)MetaCyc: 57% identical to hydroxyvaleryl-CoA reductase / acetoacetyl-CoA reductase (Cupriavidus necator)
Acetoacetyl-CoA reductase. [EC: 1.1.1.36]; 1.1.1.36 [EC: 1.1.1.36]
Predicted SEED Role
"Acetoacetyl-CoA reductase (EC 1.1.1.36)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism or Serine-glyoxylate cycle (EC 1.1.1.36)
MetaCyc Pathways
- polyhydroxybutanoate biosynthesis (3/3 steps found)
- (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
- photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
- (2S)-ethylmalonyl-CoA biosynthesis (2/4 steps found)
- acetyl-CoA fermentation to butanoate (4/7 steps found)
- 4-oxopentanoate degradation (2/9 steps found)
- ethylmalonyl-CoA pathway (3/11 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.36
Use Curated BLAST to search for 1.1.1.36
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8QN49 at UniProt or InterPro
Protein Sequence (246 amino acids)
>Dsui_2538 acetoacetyl-CoA reductase (Dechlorosoma suillum PS) MSKVALVTGALGGLGTAISQALAKEGYKVVAAYHPEFDKKEEWLAEQEAAGFKDFVLVPG DVSDYESAKAMIAEAEAKAGPIDILVNNAGITRDKFFVKMDKGQWDAVINTNLNSLFNVT HHVAAKMGERGWGRIVNISSVNGVKGQAGQTNYSAAKAGVIGFTKALAQEFAAKGVTVNA IAPGYVATKMVTAIREDILKGIIDSVPMKRLAKPEEIGAAVVYLTSELAGFVTGATLNIN GGLYYQ