Protein Info for Dsui_2531 in Dechlorosoma suillum PS
Annotation: uncharacterized protein, YfiH family
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to POLOX_PSEAE: Polyphenol oxidase (PA4543) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K05810, conserved hypothetical protein (inferred from 66% identity to dar:Daro_1605)MetaCyc: 57% identical to purine nucleoside phosphorylase YfiH (Escherichia coli K-12 substr. MG1655)
Adenosine deaminase. [EC: 3.5.4.4]; S-methyl-5'-thioadenosine phosphorylase. [EC: 3.5.4.4, 2.4.2.28]; Purine-nucleoside phosphorylase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; 2.4.2.1 [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1]; Laccase. [EC: 3.5.4.4, 2.4.2.28, 2.4.2.1, 1.10.3.2]
Predicted SEED Role
"COG1496: Uncharacterized conserved protein"
MetaCyc Pathways
- superpathway of purine nucleotide salvage (13/14 steps found)
- adenine and adenosine salvage III (4/4 steps found)
- 5'-deoxyadenosine degradation I (3/3 steps found)
- adenine and adenosine salvage I (2/2 steps found)
- S-methyl-5'-thioadenosine degradation II (1/1 steps found)
- adenine and adenosine salvage V (2/3 steps found)
- guanine and guanosine salvage I (1/2 steps found)
- xanthine and xanthosine salvage (1/2 steps found)
- adenosine nucleotides degradation II (3/5 steps found)
- inosine 5'-phosphate degradation (2/4 steps found)
- superpathway of guanine and guanosine salvage (1/3 steps found)
- purine ribonucleosides degradation (3/6 steps found)
- guanosine nucleotides degradation III (1/4 steps found)
- purine nucleotides degradation II (aerobic) (6/11 steps found)
- ureide biosynthesis (2/7 steps found)
- nucleoside and nucleotide degradation (halobacteria) (1/6 steps found)
- L-methionine salvage cycle III (4/11 steps found)
- sesamin biosynthesis (1/8 steps found)
- justicidin B biosynthesis (1/10 steps found)
- matairesinol biosynthesis (1/10 steps found)
- nucleoside and nucleotide degradation (archaea) (1/10 steps found)
- salinosporamide A biosynthesis (2/15 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from histidine and purine
- Methionine metabolism
- Nicotinate and nicotinamide metabolism
- Purine metabolism
- Pyrimidine metabolism
- Urea cycle and metabolism of amino groups
Isozymes
Compare fitness of predicted isozymes for: 2.4.2.28
Use Curated BLAST to search for 1.10.3.2 or 2.4.2.1 or 2.4.2.28 or 3.5.4.4
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8QN42 at UniProt or InterPro
Protein Sequence (249 amino acids)
>Dsui_2531 uncharacterized protein, YfiH family (Dechlorosoma suillum PS) MTKPTNSLPGFAPDWPAPPRVRSLQTTRSGGVSPAPYASLNLGDHVGDAPAHVAANRALL RAGLPGEPLWLQQVHGNRVADADSATTGREADGVVARRPGSVCAIMTADCLPVLLCDRDG TVVGAAHAGWRGLAAGVLENTVAAMGVAPERLLAWLGPAIGPQAFEVGPEVRDAFEKADP RASQAFVPGASTGKYLADIYALARLRLQAAGVREIHGGGLCTVGDAASYFSYRRDRVCGR MASLIWLEP