Protein Info for Dsui_2371 in Dechlorosoma suillum PS

Annotation: cysteine desulfurase IscS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02006: cysteine desulfurase IscS
  • amino acids 1 to 402 (402 residues), 758.5 bits, E=8.1e-233
• PF00266: Aminotran_5
  • amino acids 5 to 365 (361 residues), 309 bits, E=9.2e-96
• PF01212: Beta_elim_lyase
  • amino acids 48 to 263 (216 residues), 34.6 bits, E=2.8e-12
• PF00155: Aminotran_1_2
  • amino acids 50 to 287 (238 residues), 37.1 bits, E=4.4e-13
• PF01041: DegT_DnrJ_EryC1
  • amino acids 65 to 188 (124 residues), 37.9 bits, E=2.5e-13

Best Hits

Swiss-Prot: 88% identical to ISCS_DECAR: Cysteine desulfurase IscS (iscS) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 88% identity to dar:Daro_1950)

MetaCyc: 72% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]

Predicted SEED Role

"Cysteine desulfurase (EC 2.8.1.7), IscS subfamily" in subsystem Alanine biosynthesis or Thiamin biosynthesis (EC 2.8.1.7)

MetaCyc Pathways

• superpathway of L-alanine biosynthesis (4/4 steps found)
• L-alanine biosynthesis III (1/1 steps found)
• L-cysteine degradation IV (1/1 steps found)
• superpathway of thiamine diphosphate biosynthesis II (8/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (7/10 steps found)
• cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (4/7 steps found)
• bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
• molybdopterin biosynthesis (3/6 steps found)
• thiazole component of thiamine diphosphate biosynthesis I (3/6 steps found)
• tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
• tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
• [2Fe-2S] iron-sulfur cluster biosynthesis (3/10 steps found)
• tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
• tRNA-uridine 2-thiolation and selenation (bacteria) (3/11 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.7

Use Curated BLAST to search for 2.8.1.7

Sequence Analysis Tools

See G8QLU1 at UniProt or InterPro

Protein Sequence (402 amino acids)

>Dsui_2371 cysteine desulfurase IscS (Dechlorosoma suillum PS)
MKLPIYLDYSATTPVDPRVAEKMIPFLVEKFGNPASRSHSYGWEAEAAVEEAREQVAALV
NADPKEIVWTSGATESNNLAIKGAANFYAGKGKHIITVKTEHKAVLDTVREMERQGFSAT
YLDVQENGLIDLEVLKAAIRPDTVLVSVMFVNNEIGVVQPIAEIGEICREKGIIFHVDAA
QATGKVDIDLEKLKVDLMSFSAHKTYGPKGIGALYIRRKPRVRLEAQMHGGGHERGFRSG
TLATHQIVGMGEAFRLAKEEMVEENKRIAALRDKLLKGLSDMEAVYVNGDLEHRVAHNLN
MSFAYVEGESLLMAIKDVAVSSGSACTSASLEPSYVLRALGREDELAHSSIRFSIGRFTT
EEEVDFVVKLLHSKVGKLRELSPLWDMFKEGIDLNTVQWAAH