Protein Info for Dsui_2241 in Dechlorosoma suillum PS

Annotation: OAH/OAS sulfhydrylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01326: O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase
  • amino acids 3 to 422 (420 residues), 668.4 bits, E=1.8e-205
• PF01053: Cys_Met_Meta_PP
  • amino acids 5 to 421 (417 residues), 455.8 bits, E=1.8e-140
• PF01041: DegT_DnrJ_EryC1
  • amino acids 58 to 182 (125 residues), 34.9 bits, E=2.1e-12
• PF00155: Aminotran_1_2
  • amino acids 61 to 221 (161 residues), 31.2 bits, E=2.7e-11
• PF00266: Aminotran_5
  • amino acids 86 to 211 (126 residues), 33.8 bits, E=3.8e-12

Best Hits

Swiss-Prot: 56% identical to METY_LEPME: O-acetyl-L-homoserine sulfhydrylase (metY) from Leptospira meyeri

KEGG orthology group: K01740, O-acetylhomoserine (thiol)-lyase [EC: 2.5.1.49] (inferred from 83% identity to app:CAP2UW1_1025)

MetaCyc: 56% identical to O-acetylhomoserine sulfhydrylase (Leptospira meyeri)
O-acetylhomoserine aminocarboxypropyltransferase. [EC: 2.5.1.49]

Predicted SEED Role

"O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48)" in subsystem Methionine Biosynthesis (EC 2.5.1.48, EC 2.5.1.49)

MetaCyc Pathways

• aspartate superpathway (22/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (15/18 steps found)
• superpathway of L-methionine biosynthesis (by sulfhydrylation) (10/12 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
• L-homocysteine biosynthesis (2/2 steps found)
• L-methionine biosynthesis III (3/4 steps found)
• homocysteine and cysteine interconversion (3/4 steps found)
• L-methionine biosynthesis II (4/6 steps found)
• superpathway of S-adenosyl-L-methionine biosynthesis (6/9 steps found)
• superpathway of L-methionine biosynthesis (transsulfuration) (6/9 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation III (3/5 steps found)
• superpathway of L-homoserine and L-methionine biosynthesis (5/8 steps found)
• S-methyl-5-thio-α-D-ribose 1-phosphate degradation II (2/5 steps found)
• L-methionine biosynthesis I (2/5 steps found)

KEGG Metabolic Maps

• Biosynthesis of plant hormones
• Cysteine metabolism
• Methionine metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.48, 2.5.1.49

Use Curated BLAST to search for 2.5.1.48 or 2.5.1.49

Sequence Analysis Tools

See G8QKC1 at UniProt or InterPro

Protein Sequence (424 amino acids)

>Dsui_2241 OAH/OAS sulfhydrylase (Dechlorosoma suillum PS)
MPKLETLAVHAGYSPDPTTKAAAVPIYQTTSYAFDNTQHGADLFDLKVAGNIYTRIMNPT
QDVLEKRVAALEGGIAGLALASGQAAITYAIQTIAEAGDNIVSTATLYGGTYNLFAHTFP
QLGIQVRFADYRDPASFEKLIDGKTKAIYAETIGNPLGNITDIEKLAEIAHRNGIPLIVD
NTVASPFLLRPIEFGADIVVHSLTKYLGGHGNSIGGIIVDSGKFPWAEHKTRFKRLNEPD
VSYHGVVYTEALGPAAYIGRARVVPLRNTGAAISPFNAFLILQGIETAALRMERITDNTI
AVAKYLQSHKKVKWVNYAGLENHPDHALAKKYLGGKPSGILTFGVEGGIEGGTRFQDALQ
LFTRLVNIGDAKSLACHPASTTHRQLNPEELAKAGVTVDTVRLSIGIEHIDDLIADLEQA
LAAV