Protein Info for Dsui_2175 in Dechlorosoma suillum PS

Annotation: 2,3-bisphosphoglycerate-independent phosphoglycerate mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 546 TIGR01307: phosphoglycerate mutase (2,3-diphosphoglycerate-independent)" amino acids 14 to 539 (526 residues), 438.1 bits, see alignment E=1.8e-135 PF01676: Metalloenzyme" amino acids 14 to 533 (520 residues), 233.8 bits, see alignment E=2.3e-73 PF06415: iPGM_N" amino acids 94 to 314 (221 residues), 221.8 bits, see alignment E=7.9e-70

Best Hits

Swiss-Prot: 57% identical to GPMI_LEPIC: Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) from Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni (strain Fiocruz L1-130)

KEGG orthology group: K01834, phosphoglycerate mutase [EC: 5.4.2.1] (inferred from 86% identity to dar:Daro_0233)

Predicted SEED Role

"2,3-bisphosphoglycerate-independent phosphoglycerate mutase (EC 5.4.2.1)" in subsystem Entner-Doudoroff Pathway or Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes (EC 5.4.2.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 5.4.2.1

Use Curated BLAST to search for 5.4.2.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QJR7 at UniProt or InterPro

Protein Sequence (546 amino acids)

>Dsui_2175 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (Dechlorosoma suillum PS)
MLQKLSSFPGVPGPVVIIVMDGYGLPKTDVGSAIAAARKPTLDKLFATCPNIRLKAHGTA
VGMPSDDDMGNSEVGHNAIGAGQVYAQGAALVAGAIADGSIWQGQAWQEIVAGAKAGRGV
LHFIGLFSDGNVHSHIDHLKAMVLQAKAEGVKTVRIHALLDGRDVPETSALEYVEPFEAF
LAEISDPSFDARIASGGGRQVITMDRYEANWALVEAGWKTHVLGQGEQFASTSEAVKALR
QRHPGTIDQDLPPFVIGANGQPVGTIEDGDAVVFYNFRGDRAIEITRAFEEADFDKFDRI
RTPKVTYAGMLQYDGDLQLPKRFLVAPPAIKDTMGEWFAKSGVTQFACSETQKFGHVTYF
WNGNRSGKFEGETYQEVPSDVVPFEQRPWMKSAEIADAMIAALKSGQYKTLRCNFANGDM
VGHTGNFRAATLSVEAVDLALERILPVIDAMGGVALITADHGNADEMFELDKKTKQPQLN
ADGSFKAKTAHTLNPVPLILYDNVSGGKLALKQTESAGLSNIAATVANLLGYEKHAKWDE
SLLQIR