Protein Info for Dsui_2149 in Dechlorosoma suillum PS

Annotation: DNA helicase, Rad3

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 PF00270: DEAD" amino acids 40 to 92 (53 residues), 27.4 bits, see alignment 3.9e-10 PF13307: Helicase_C_2" amino acids 469 to 630 (162 residues), 151.7 bits, see alignment E=3.1e-48

Best Hits

KEGG orthology group: K03722, ATP-dependent DNA helicase DinG [EC: 3.6.4.12] (inferred from 72% identity to app:CAP2UW1_3275)

Predicted SEED Role

"DinG family ATP-dependent helicase YoaA" in subsystem DNA repair, bacterial DinG and relatives

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QJP1 at UniProt or InterPro

Protein Sequence (650 amino acids)

>Dsui_2149 DNA helicase, Rad3 (Dechlorosoma suillum PS)
MLASPHLVADTAAVFEPGGPLSANIPGYRTRSQQVEMAEKIAAAIADNRMLVAEAGTGTG
KTYAYLVPALLSGGKVIVSTGTKTLQDQLFNKDIPTVRAALKAPVKVALLKGRANYVCPY
HLDRSLLDGRFQNREDVAYIQKIARFAKETRSGDKAECLDVPENATAWIMATSTRENCLG
QECPHHKECFVLQARKEALSADVVVVNHHLFFADVMLKDEGMAELLPACNAVIFDEAHQL
PETASLFFGDSFSTSQVLDLARDARSEGLASARDCLDLQEATRIVDKAAKDLRLTVAVEP
ARYAWGQLEGNAQFLDGLANLEKELELLNAILENQAERSEGLENCWRRNGELLQRLIQWR
QEQPGVVRWGEAFNHSLQLNATPLDVARLFRKQLDGHPRAWIFTSATLAVQADFSHYCNE
LGLWDAESARWESPFDYGQQAVLYAPRDIPDPNLPGFTEAVVEAAWPALKACGGGAFFLC
TSLRAMRRTHELLAAKLEDEGLEMPLLLQGEGSKTELLERFRRLGNAVLVGSQSFWEGVD
VRGEALSLVVIDKLPFAPPDDPVLKARVEQMEKEGRSAFMEYQLPRAVINVKQGAGRLIR
DETDRGVLMICDPRLITKPYGKRVWRSLPPMKRTRDLIEVLDFYAALQPN