Protein Info for Dsui_2042 in Dechlorosoma suillum PS

Annotation: cystathionine beta-lyase/cystathionine gamma-synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01053: Cys_Met_Meta_PP
  • amino acids 9 to 392 (384 residues), 499.1 bits, E=3.1e-154

Best Hits

Swiss-Prot: 61% identical to METC_COXBU: Cystathionine beta-lyase (metC) from Coxiella burnetii (strain RSA 493 / Nine Mile phase I)

KEGG orthology group: K01758, cystathionine gamma-lyase [EC: 4.4.1.1] (inferred from 66% identity to rce:RC1_1986)

MetaCyc: 53% identical to cystathionine gamma-lyase monomer (Homo sapiens)
Homocysteine desulfhydrase. [EC: 4.4.1.2]; Cystathionine gamma-lyase. [EC: 4.4.1.2, 4.4.1.1, 4.4.1.28]; 4.4.1.1 [EC: 4.4.1.2, 4.4.1.1, 4.4.1.28]; Cystathionine beta-synthase. [EC: 4.4.1.2, 4.4.1.1, 4.4.1.28, 4.2.1.22]; 4.2.1.22 [EC: 4.4.1.2, 4.4.1.1, 4.4.1.28, 4.2.1.22]; RXN-14632 [EC: 4.4.1.2, 4.4.1.1, 4.4.1.28, 4.2.1.22]

Predicted SEED Role

"Cystathionine gamma-lyase (EC 4.4.1.1)" in subsystem Cysteine Biosynthesis or Glycine and Serine Utilization or Methionine Biosynthesis or Methionine Degradation (EC 4.4.1.1)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• hydrogen sulfide biosynthesis II (mammalian) (6/6 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (12/15 steps found)
• L-cysteine degradation II (3/3 steps found)
• superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae) (8/10 steps found)
• L-threonine degradation I (5/6 steps found)
• superpathway of L-cysteine biosynthesis (fungi) (5/6 steps found)
• L-cysteine biosynthesis III (from L-homocysteine) (2/2 steps found)
• superpathway of L-cysteine biosynthesis (mammalian) (4/5 steps found)
• homocysteine and cysteine interconversion (3/4 steps found)
• D-serine degradation (2/3 steps found)
• L-methionine degradation II (2/3 steps found)
• L-serine degradation (2/3 steps found)
• L-tryptophan degradation II (via pyruvate) (2/3 steps found)
• L-methionine biosynthesis II (4/6 steps found)
• taurine biosynthesis III (1/3 steps found)
• felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
• L-mimosine degradation (4/8 steps found)
• superpathway of L-threonine metabolism (11/18 steps found)
• L-cysteine biosynthesis VI (reverse transsulfuration) (3/7 steps found)
• glycine betaine degradation III (3/7 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• glycine betaine degradation I (3/8 steps found)
• hypoglycin biosynthesis (4/14 steps found)
• purine nucleobases degradation II (anaerobic) (10/24 steps found)

KEGG Metabolic Maps

• Cysteine metabolism
• Glycine, serine and threonine metabolism
• Methionine metabolism
• Nitrogen metabolism
• Selenoamino acid metabolism
• Sulfur metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.22, 4.4.1.1

Use Curated BLAST to search for 4.2.1.22 or 4.4.1.1 or 4.4.1.2 or 4.4.1.28

Sequence Analysis Tools

See G8QIK1 at UniProt or InterPro

Protein Sequence (392 amino acids)

>Dsui_2042 cystathionine beta-lyase/cystathionine gamma-synthase (Dechlorosoma suillum PS)
MTHKQGFATRAIHAGQEPDPTTGAVSIPIYATSTYVQPALNEPKQYAGATFDYARSANPT
RLALERCIADLEGGAFATATGFAFASGLAASATVLELLPAGSHVVASDDLYGGSVRLFNR
VRSNSADLKVTYADFSSRESIEAAITPETKLLWVETPSNPLLKLVDLDLVAAIGKDRGLL
TVADNTFASPWIQRPLEHGFDIVVHSATKYLNGHSDVIAGVAVTARADLAERLAFLQNAA
GAVLGPFDAFLALRGLKTLALRMERHCANAEAIARWLEQHPAIEKVHYPGLASHPQHELA
KRQMRGFGGMVSAVVKGGLPAAKKLLENTKIFVLAESLGGVESLIELPALMTHASVPPEQ
RRRIGIDDGLVRLSVGVEDVEDLLADLEQALK