Protein Info for Dsui_1938 in Dechlorosoma suillum PS

Annotation: DNA gyrase, A subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 873 TIGR01063: DNA gyrase, A subunit" amino acids 10 to 841 (832 residues), 1220.3 bits, see alignment E=0 PF00521: DNA_topoisoIV" amino acids 32 to 506 (475 residues), 558.9 bits, see alignment E=8.4e-172 PF03989: DNA_gyraseA_C" amino acids 538 to 585 (48 residues), 42.7 bits, see alignment 2.9e-15 amino acids 588 to 638 (51 residues), 39.9 bits, see alignment 2.2e-14 amino acids 645 to 688 (44 residues), 31 bits, see alignment 1.4e-11 amino acids 693 to 738 (46 residues), 41.7 bits, see alignment 6.2e-15 amino acids 744 to 790 (47 residues), 47.3 bits, see alignment 1.1e-16 amino acids 794 to 840 (47 residues), 45.1 bits, see alignment 5.6e-16

Best Hits

Swiss-Prot: 60% identical to GYRA_SALTY: DNA gyrase subunit A (gyrA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K02469, DNA gyrase subunit A [EC: 5.99.1.3] (inferred from 82% identity to app:CAP2UW1_2904)

MetaCyc: 59% identical to DNA gyrase subunit A (Escherichia coli K-12 substr. MG1655)
5.99.1.3-RXN [EC: 5.6.2.2]

Predicted SEED Role

"DNA gyrase subunit A (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.6.2.2 or 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QHV8 at UniProt or InterPro

Protein Sequence (873 amino acids)

>Dsui_1938 DNA gyrase, A subunit (Dechlorosoma suillum PS)
MDQFAKETLPISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPVHRRVLFAMHELGNDWN
KAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQDFSLRYMLVDGQGNFGSVDGDNAAAM
RYTEVRMARIGHQLLEDIDKETVDFGPNYDGSEQEPLILPARIPNLLINGSSGIAVGMAT
NIPPHNLGEVVAACLALLQNPNLTIDELIDIIPAPDFPTAGIIYGVAGVREGYRTGRGRV
VMRAKTHVEDLDKGNRQALIIDELPYQVNKRTLLEKIGELVNEKRIEGISDLRDESDKSG
MRVVIELKRGEMPEVVLNNLFKMTQLQDTFGMNMVALVDGQPRLLNLKQMLECFLAHRRE
VLNRRTVFELRKAREKAHTQEGLAVALDNVDEIIALIKAAPTPADARRELMARMWVSATV
TEMLARASLDATRPEGIAPEYGLSAQGYRLTEAQAQAILELRLQRLTGLERDKIVADYKE
LLEKIADLMDILARPERVTEIIGEELTAIRDQFGDDRRSEIVAYAQDLGIEDLITPQDMV
VTLSHTGYIKSQPLADYRAQRRGGRGKQAAAMKDDDFVDHLFVANTHDYILCFSNRGRCY
WLKVYEVPQGSRISRGKPIINMFPMEEGEQINAILPVKEFSDDKYVFMCTVQGTVKKTSL
ANFSNPRKAGIIAVDLDEGDYLIGADLTTGQSDIVLVSDGGKAVWFGEDQVRPMGRQSRG
VRGMMLQEGQSVISLLVAESEEETVLVATENGYGKRTRLGDFRRSSRGTQGVMAIAASER
NGKIVAAKLVTDADEVMLITTGGVLIRTRVAEIRELGRATQGVTLISLDAGEKLSGLEKV
AESEGDTDAEAGDELADGEMLEGGEDSAASEEA