Protein Info for Dsui_1925 in Dechlorosoma suillum PS

Annotation: acetolactate synthase, large subunit, biosynthetic type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00118: acetolactate synthase, large subunit, biosynthetic type
  • amino acids 21 to 571 (551 residues), 685.2 bits, E=3.1e-210
• PF02776: TPP_enzyme_N
  • amino acids 22 to 136 (115 residues), 141.9 bits, E=1.2e-45
• PF00205: TPP_enzyme_M
  • amino acids 209 to 344 (136 residues), 161.3 bits, E=1.6e-51
• PF02775: TPP_enzyme_C
  • amino acids 400 to 547 (148 residues), 177.7 bits, E=2e-56

Best Hits

Swiss-Prot: 65% identical to ILVB_ECOLI: Acetolactate synthase isozyme 1 large subunit (ilvB) from Escherichia coli (strain K12)

KEGG orthology group: K01652, acetolactate synthase I/II/III large subunit [EC: 2.2.1.6] (inferred from 77% identity to app:CAP2UW1_0276)

MetaCyc: 65% identical to acetohydroxy acid synthase I subunit IlvB (Escherichia coli K-12 substr. MG1655)
Acetolactate synthase. [EC: 2.2.1.6]; 2.2.1.6 [EC: 2.2.1.6]

Predicted SEED Role

"Acetolactate synthase large subunit (EC 2.2.1.6)" in subsystem Acetoin, butanediol metabolism or Branched-Chain Amino Acid Biosynthesis (EC 2.2.1.6)

MetaCyc Pathways

• superpathway of branched chain amino acid biosynthesis (17/17 steps found)
• superpathway of L-isoleucine biosynthesis I (13/13 steps found)
• L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
• L-valine biosynthesis (4/4 steps found)
• pyruvate fermentation to isobutanol (engineered) (4/5 steps found)
• pyruvate fermentation to (R)-acetoin I (2/3 steps found)
• pyruvate fermentation to (S)-acetoin (2/3 steps found)
• L-isoleucine biosynthesis IV (4/6 steps found)
• pyruvate fermentation to (R)-acetoin II (1/2 steps found)
• L-isoleucine biosynthesis II (5/8 steps found)
• L-isoleucine biosynthesis III (4/7 steps found)
• superpathway of (R,R)-butanediol biosynthesis (2/5 steps found)
• superpathway of L-threonine metabolism (11/18 steps found)
• superpathway of 2,3-butanediol biosynthesis (2/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Butanoate metabolism
• C5-Branched dibasic acid metabolism
• Pantothenate and CoA biosynthesis
• Valine, leucine and isoleucine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 2.2.1.6

Use Curated BLAST to search for 2.2.1.6

Sequence Analysis Tools

See G8QHU5 at UniProt or InterPro

Protein Sequence (572 amino acids)

>Dsui_1925 acetolactate synthase, large subunit, biosynthetic type (Dechlorosoma suillum PS)
MNAPAPQAARQTDAAPAPETLTGAQIVVRLLERQGVTTVSGIPGGAILPFYDALSASEKI
RHVLARHEQGAGFIAQGMARASGQPAVCLASSGPGATNLLTAIADAKLDSIPLVAITGQV
PRSMIGTDAFQEVDTYGLSIPITKHNFLVNSAEELLEVIPRAFTLAASGRPGPVLVDIPK
DVQAQAIEVSQWPAPGGRQAPPAPDMAAIEKAAAMINAAERPILYLGGGVVHSGAAEAAV
ALAEKASLPTTMTLMALGAMPVDHPLSIHMLGMHGARYTNLLLEECDLLIAVGARFDDRA
TGKVAAFCPKAQIIHIDIDPSELDKIKNAHVGIAGDVGEVLEKLSPLVEAHLRKRWLTHM
GDLRSRFRLQRPNLEDPRSHYGLIQAVADCLDDSAAIATDVGQHQMWVAQAYPLRRPRQW
LTSGGLGTMGFGVPAALGAALAEPERTVVCFTGDGSILMNIQELATIAEAGANLKIVLMN
NDALGLVYQQQELFYGKRYFASKFAGQPDFLKIAEGFGIDTLDLDTAADPRAALRQALQR
PGPCLIHAFIDMNEKVYPMVPPGAANTDMIGG