Protein Info for Dsui_1825 in Dechlorosoma suillum PS

Annotation: NAD(P)H-dependent nitrite reductase, large subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 PF07992: Pyr_redox_2" amino acids 5 to 283 (279 residues), 197.5 bits, see alignment E=1.3e-61 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 6 to 800 (795 residues), 965.3 bits, see alignment E=1.5e-294 PF13738: Pyr_redox_3" amino acids 72 to 278 (207 residues), 32.3 bits, see alignment E=2.7e-11 PF00070: Pyr_redox" amino acids 147 to 229 (83 residues), 66.3 bits, see alignment E=1.1e-21 PF18267: Rubredoxin_C" amino acids 319 to 386 (68 residues), 64.6 bits, see alignment E=2.6e-21 PF04324: Fer2_BFD" amino acids 423 to 471 (49 residues), 54.9 bits, see alignment 3.7e-18 amino acids 488 to 538 (51 residues), 48.8 bits, see alignment 2.9e-16 PF03460: NIR_SIR_ferr" amino acids 563 to 626 (64 residues), 58.4 bits, see alignment 2e-19 PF01077: NIR_SIR" amino acids 636 to 774 (139 residues), 111.1 bits, see alignment E=1.5e-35

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 79% identity to dar:Daro_0816)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QGR5 at UniProt or InterPro

Protein Sequence (812 amino acids)

>Dsui_1825 NAD(P)H-dependent nitrite reductase, large subunit (Dechlorosoma suillum PS)
MNKPKLVLVGNGMAGVRTLEELLKIAPDHYDITVFGAEPHPNYNRILLSPVLAGEMTVPE
IVLNDLQWYADNGIKLHLNKKVTKVDRVRRQVVAEDGTVESYDRLLLATGSNPFMLPIPG
NDLPGVIAYRDIADTDAMIEAARTHRHAVVIGGGLLGLEAANGLKLRGMDVTVVHVGPWL
LERQLDEVAGRMLQQSLEERGLKFLLQKNTEALIAGESGRVAAIRFKDGMQIPADLVVMA
VGIRPNTALAESSGIHCNRGIVVSDTLQTYDPKIYAVGECVSHRGIAYGLVAPLFEQAKV
CANHLAGYGIGRYQGSVTSTKLKVTGIDLFSAGDFMGGSDTEDIVLHDPAGGVYKRLVLK
DNKLVGGVLYGDTADGSWYFQLLREGKDVSQIRDHLMFGQSNLGDTGHQGHNKAAAMADS
AEVCGCNGVTKGVIVKAIKEKGLFTLDEVRKHTKASASCGSCTGLVEQILASTVGGAYQA
ASSAEKPMCGCTEHSHGAVRQAIRERHLTSIPAVIEALAWKNPNGCEKCRPALNYYLIST
FPHEAMDDPQSRFINERAHANIQKDGTYSVVPRMWGGVTTPNELRAIADVADKYQVPMVK
VTGGQRIDLLGVKKEDLPHMWADLAQAGMVSGHAYGKSIRTVKTCVGSQFCRFGTQDSTG
MGIELEKMLFDMWSPHKVKLAVSGCPRNCAESGIKDVGIIAVDSGWEIHVAGNGGIKTEA
AQFLCKVATAEEVKEYSGAFLQLYREEGFYLERTVHYVQRVGLDHVKRLVVEDEDNRKAL
HARLLDSLKDAADPWAEHQEAPARREFEPLAF