Protein Info for Dsui_1257 in Dechlorosoma suillum PS

Annotation: DNA-directed DNA polymerase III PolC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1151 TIGR00594: DNA polymerase III, alpha subunit" amino acids 6 to 1024 (1019 residues), 1185.4 bits, see alignment E=0 PF02811: PHP" amino acids 8 to 174 (167 residues), 136 bits, see alignment E=4.8e-43 PF07733: DNA_pol3_alpha" amino acids 292 to 562 (271 residues), 342 bits, see alignment E=7.7e-106 PF17657: DNA_pol3_finger" amino acids 565 to 730 (166 residues), 215.6 bits, see alignment E=8e-68 PF14579: HHH_6" amino acids 803 to 892 (90 residues), 109.1 bits, see alignment 3.1e-35 PF01336: tRNA_anti-codon" amino acids 989 to 1065 (77 residues), 34 bits, see alignment 7e-12 PF20914: DNA_pol_IIIA_C" amino acids 1090 to 1139 (50 residues), 32.9 bits, see alignment (E = 1.4e-11)

Best Hits

Swiss-Prot: 55% identical to DPO3A_NEIMA: DNA polymerase III subunit alpha (dnaE) from Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)

KEGG orthology group: K02337, DNA polymerase III subunit alpha [EC: 2.7.7.7] (inferred from 71% identity to eba:ebA4398)

Predicted SEED Role

"DNA polymerase III alpha subunit (EC 2.7.7.7)" in subsystem DNA-replication (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QLR7 at UniProt or InterPro

Protein Sequence (1151 amino acids)

>Dsui_1257 DNA-directed DNA polymerase III PolC (Dechlorosoma suillum PS)
MTDPRFVHLRLHSEYSITDGIVRLDDAVARAIADGQPAMALTDLANAFGLVKFYNACRGK
GVKPVAGADVWIANETETDRPFRLLLLVRSHRGYLQLCELLSRAYLAEGRRDRAEIKRAW
FDEVGCDGLIALSGAKDGDVGDALLAGNRDLAAARAKAWSAQFPQAFYLEVQRAGHPQQE
QLVSATADLGADLELPLVATHPIQFLDADDFKAHEARVCIAEGYVLGDRRRPKTFTEQQY
FKTQAEMAELFADLPEALENTLEIAKRCNLTLTLGKNFLPQFPTPDGISLDQHLINEAKA
GLEKRLELLFPDPEERQRRRPEYDERLEIETKTIVQMGFPGYFLIVADFINWGKHNGVPV
GPGRGSGAGSLVAYSLGITDLDPLQYALLFERFLNPERVSMPDFDIDFCQDNRWRVIEYV
RHKYGVDAVSQIATFGTMSSKAVIRDVGRVLDLPYNFCDSLSKLIPVEANKPVSLAQALE
MEPQLKEKMEEEEEVAELFELAMKLEDLTRNVGMHAGGVLIAPGKLTDFCPLYAQPGSDS
VVSQYDKDDVEKVGLVKFDFLGLRNLTIIELAVEYVERLTGEKLDLLSLPFTDPAAYQIL
KDANTTAIFQVESEGMKKLLKKLAPDRFEDIIAVLALYRPGPLGSGMVDDFILRKKGQQK
IDYFHPDLKGCLDPTYGVIVYQEQVMQISQIIGGYTLGGADMLRRAMGKKKAEEMAKHRE
TIAEGAKKKGYDPALAEQLFDLMTKFAEYGFNKSHTAAYAMVTYHTAWLKAHHCSAFMAA
TMSSDMDNTDTVKIFYEDTVKNGIKVLPPDVNHSDYRFVPVDGKTIRYGLGAVKGTGQQA
VECILAARAEGGPFKDLFDFCLRVDKRLVNRRTIEALIRAGAFDTLDPSRDRAQLIASVS
IAMEAADQAERNAHQGGLFEMFGSGDDAVAEAPQYVSVRPWSDKQRLLEEKTALGFFYSG
HPFDSIRSEAKRFAGRPLSALAPAREPQTLAGVVMDLRVKMTSRGRMGIIILDDGSCPSL
EVTVYSEVFDAHRNKLKVDEPLIIEGKVSKDDFRDGLRIVAENLYTLPEARTRFARQLRL
SMNGQADARKLQELLTPFRMEGGCPVRIAYRNGEAQCELILGEGTRVRPDDDLLQTLQDW
LSPAGVELQYN