Protein Info for Dsui_1194 in Dechlorosoma suillum PS

Annotation: tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 436 (433 residues), 499.4 bits, see alignment E=8.3e-154 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 4 to 439 (436 residues), 606.8 bits, see alignment E=2.7e-186 PF00919: UPF0004" amino acids 4 to 104 (101 residues), 103.9 bits, see alignment E=6e-34 PF04055: Radical_SAM" amino acids 151 to 324 (174 residues), 99.1 bits, see alignment E=5e-32 PF01938: TRAM" amino acids 377 to 439 (63 residues), 58.7 bits, see alignment E=6.4e-20

Best Hits

Swiss-Prot: 85% identical to MIAB_DECAR: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Dechloromonas aromatica (strain RCB)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 85% identity to dar:Daro_3527)

MetaCyc: 64% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QKZ0 at UniProt or InterPro

Protein Sequence (446 amino acids)

>Dsui_1194 tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB (Dechlorosoma suillum PS)
MSKKLYIKTFGCQMNEYDSDKMADVLNQSEPLEKTDNPEEADIILFNTCSVREKAQEKVF
HDLGRVKHLKKLNPNLVIGVGGCVASQEGAAIVARAPYVDVVFGPQTLHRLPQLIAERKS
QGKAAVDVSFPEIEKFDALPPAKVEGATAFVSIMEGCSKFCTFCIVPYTRGAEVSRPFED
ILTEVAGLAQQGVKEVTLLGQNVNAYRGEFGGEVADLAMLIEYIAEMPGIERIRYTTSHP
REMTQRLIDTYAKVPKLVSHLHLPVQAGADRVLAVMKRGYTAIEYKSIIRKLRVARPDIS
LSSDFIVGFPGETEEDFEKTMKLIEDVGFDTSFSFVYSPRPGTPAVELEDDTPQDVKLAR
LARLQKRIEEQAMAISQAMVGTVQRVLVEGTSKKDANELAGRTDNNRVVNFVGNPRLINH
FVDVTITSALPHSLRGEIVTREGVAA