Protein Info for Dsui_1096 in Dechlorosoma suillum PS
Annotation: glutamate synthase family protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00284, glutamate synthase (ferredoxin) [EC: 1.4.7.1] (inferred from 68% identity to aav:Aave_1008)Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- L-glutamate and L-glutamine biosynthesis (6/7 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- superpathway of ammonia assimilation (plants) (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle II (2/2 steps found)
- L-glutamate biosynthesis V (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- nitrogen remobilization from senescing leaves (6/8 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (3/4 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- GABA shunt II (1/4 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.7.1
Use Curated BLAST to search for 1.4.1.13 or 1.4.7.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8QK65 at UniProt or InterPro
Protein Sequence (1565 amino acids)
>Dsui_1096 glutamate synthase family protein (Dechlorosoma suillum PS) MDSVVPGGAVPGRQGLYDPANEKDACGVGFVAHIKNKKSHGIVEQGLLILKNLDHRGATG YDPLLGDGAGILIQMPDAFLRAEAAKLGINLPKEGEYACGIVFLPQSSNGRAAAESAVAR IVHEEGQTFLGWRDVPRDNAGLAAAAREIEPVMRQVFIAKGENVGDQDAFERKLFVIRKR VEHAVRKLKLDDGKQFYIPSLSSRTINYKGMLLAHQVGEYYLDLQDERLVSALALVHQRF STNTFPTWDLAHPFRMIAHNGEINTVRGNVNWMKARQKAMSSKWLKEDLDKLWPLIVDGQ SDSACFDNALELLVMGGYSMAQAMMMLIPEAWAGNPLMDEERRAFYEYHAALMEPWDGPA AVAFTDGRQIGATLDRNGLRPARYLVTEDDLVLMASEMGVLTFPQEKIVKKWRLQPGKMF LIDMEQGRIIDDSELKKSLSGAKPYRKWIEESRYFLGDLPEAGSEAPKADVPLLDQQQAF GFTQEDVKFILQPMAAQGEEATGSMGNDSPLTVLSNKEKPLYNYFKQLFAQVTNPPIDPI REEIVMSLTSFIGPKPNLLGVDETNPPLRLEVHQPVLTNADLAKLRAIDRSTSGRYKSLV LDITWPAAEGAAGCEKALEAVCAAAEKSVADGYNVLILSDRGVCAEKAPIPALLATSAVH HHLVKKGLRTSTGLVVDTGSARETHHFALLAGYGAEAVCPWLTMDTLAEIAGNLPGNVTA YDAQKRFIKAVNKGLNKVMSKMGISTYQSYCGAQIFEAIGLSSELVKKYFAGTATQVEGI GILQVAEEALRVHRAAFSSDPVLAESLETGGEYAFRVRGEDHMWTPDAIAKLQHSTRSGK MDTYKEYAKIINDQSKRHLTLRGLFEIKSAGAPVPLEEVEPAKEIVKRFATGAMSLGSIS TEAHTTLSVAMNRIGGKSNTGEGGEDANRFKPVKAGQKLSEIIGAGRIARDLELKEGDSL RSAIKQVASGRFGVTAEYLANADQIQIKMAQGAKPGEGGQLPGHKVSEYIGALRHSVPGV GLISPPPHHDIYSIEDLAQLIHDLKNVNPVSSISVKLVSEVGVGTVAAGVSKAKADHLVI AGHDGGTGASPLSSIKHAGTPWELGLAETQQTLVLNRLRGRIRVQVDGQMKTGRDVVIGA LLGADEFGFATAPLVVEGCIMMRKCHLNTCPVGVATQDPTLRAKFSGQPEHVVNYFFFVA EEVREIMASMGIRKLDDLIGRADLLDMRAGVEHWKARGLDYSKIFYMPPVGADVARLHCE TQDHNLGKALDHKLIEQAKPALEKGEKVVINTEIINVNRTAGAMLAGEVAKRYGHAGLPD DTIHVNLTGTAGQSFGAFLAKGVTFELTGEGNDYVGKGLSGGRIVIKAPKAFRGNTAENI IVGNTVLYGAIEGEVFFNGVGGERFCVRNSGATAVVEGVGDHGCEYMTGGTVVVLGQTGR NFAAGMSGGVAYVLDEDGTFESRCNLAQVALEPVPEEEAASEKLGEFGDVTSHGKVDIRH LAMADEALLKGLVERHLEMTGSTRAKQILDGWSTYRAKFVKVMPNEYRRALAELAAKNSK QKEAA