Protein Info for Dsui_0982 in Dechlorosoma suillum PS

Annotation: acetyl/propionyl-CoA carboxylase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 667 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 158.3 bits, see alignment E=3e-50 PF02786: CPSase_L_D2" amino acids 116 to 322 (207 residues), 260.3 bits, see alignment E=4.4e-81 PF02222: ATP-grasp" amino acids 132 to 292 (161 residues), 40.8 bits, see alignment E=6.8e-14 PF07478: Dala_Dala_lig_C" amino acids 139 to 291 (153 residues), 42 bits, see alignment E=2.7e-14 PF02785: Biotin_carb_C" amino acids 336 to 444 (109 residues), 130.9 bits, see alignment E=7.3e-42 PF21139: BT_MCC_alpha" amino acids 458 to 583 (126 residues), 48.6 bits, see alignment E=3.6e-16 PF00364: Biotin_lipoyl" amino acids 597 to 660 (64 residues), 42.6 bits, see alignment E=1.5e-14

Best Hits

KEGG orthology group: K01968, 3-methylcrotonyl-CoA carboxylase alpha subunit [EC: 6.4.1.4] (inferred from 79% identity to dar:Daro_0089)

Predicted SEED Role

"Methylcrotonyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.4)" in subsystem HMG CoA Synthesis or Leucine Degradation and HMG-CoA Metabolism or Serine-glyoxylate cycle (EC 6.4.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.4

Use Curated BLAST to search for 6.4.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QJ19 at UniProt or InterPro

Protein Sequence (667 amino acids)

>Dsui_0982 acetyl/propionyl-CoA carboxylase, alpha subunit (Dechlorosoma suillum PS)
MFSKILIANRGEIACRVIKTARRLGIRTVAVYSEADANARHVRLADEAVLLGPAAARESY
LVAEKILDAARRTGAQAIHPGYGFLSENADFAEACAAAGVVFIGPPASAIRAMGSKSAAK
ALMEKAAVPLTPGYHGDNQEPEFLKQQADAIGYPVLIKAAAGGGGKGMRLVDKGEDFIAA
LASCQREARSSFGNDQVLVEKYITRPRHIEIQVFGDSQGNCVYLFERDCSVQRRHQKVLE
EAPAPGMTPERRRQMGEAAVAAAKAVGYVGAGTVEFIANQDGSFYFMEMNTRLQVEHPVT
EMITGQDLVEWQLRVAAGEALPLRQEQLQIRGHALEARIYAEDAGKGFLPSTGRLLHLVP
PAEGLNVRVDTGVEEGDEITPHYDPMIAKLIVWDEDREAALARMRQALADYRVVGVTTNI
DFLSRLVSCPAFAGADLDTGLIERQQDFLFPESPEVPRDVILTATVAELLRERDLAAQQG
RRSGDPWSPWNRRDGWRMNIAARRTVSFRVGETQVDVGVAYAGEDWQLTLRNDTLLVRGR
LLAHDRLAVELEDRRLMASVVAVAEKRHVFLNSGTYVIERHDPLHLVEAGGAQGGGLTAP
MPGKVVALLAQPGPVAKGTPLLILEAMKMEHTITAPKQGNLKGFRYAVGEQVADGAELVD
FAPEAAA