Protein Info for Dsui_0956 in Dechlorosoma suillum PS

Annotation: nitrogenase molybdenum-iron protein alpha chain

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 480 TIGR01282: nitrogenase molybdenum-iron protein alpha chain" amino acids 14 to 478 (465 residues), 884.4 bits, see alignment E=1.6e-270 TIGR01862: nitrogenase component I, alpha chain" amino acids 30 to 473 (444 residues), 630.9 bits, see alignment E=9.9e-194 PF00148: Oxidored_nitro" amino acids 62 to 466 (405 residues), 345.3 bits, see alignment E=2.3e-107

Best Hits

Swiss-Prot: 80% identical to NIFD_BRASP: Nitrogenase molybdenum-iron protein alpha chain (nifD) from Bradyrhizobium sp. (strain ANU 289)

KEGG orthology group: K02586, nitrogenase molybdenum-iron protein alpha chain [EC: 1.18.6.1] (inferred from 83% identity to mca:MCA0230)

MetaCyc: 70% identical to [FeMo]-nitrogenase complex dinitrogenase component alpha subunit (Klebsiella pneumoniae)
Nitrogenase (flavodoxin). [EC: 1.19.6.1]

Predicted SEED Role

"Nitrogenase (molybdenum-iron) alpha chain (EC 1.18.6.1)" in subsystem Nitrogen fixation (EC 1.18.6.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.18.6.1

Use Curated BLAST to search for 1.18.6.1 or 1.19.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QIZ3 at UniProt or InterPro

Protein Sequence (480 amino acids)

>Dsui_0956 nitrogenase molybdenum-iron protein alpha chain (Dechlorosoma suillum PS)
MSAVLDDKTRNQALIQEVLEAYPEKAAKKRAKHLNVYEEGKPDCGVKSNIKSLPGVMTIR
GCAYAGSKGVVWGPIKDMVHISHGPVGCGQYSWGTRRNYASGTVGVDNFTVLQVTSDFQE
KDIVFGGDKKLDKVIDEITEIFPLARGISIQSECPIGLIGDDIEAVAKKKTKEISKTIVP
VRCEGFRGVSQSLGHHIANDMVRDWVLDKADNKGFESTPYDVTIIGDYNIGGDAWSSRIL
LEEMGLRVIAQWSGDGTLAEMEKTPKAKLNLLHCYRSMNYISRHMEEKYGIPWVEYNFFG
PSKIAASLREIASHFDDTIKENAEKVIAKYQAMVDGIIAKYKPRLTGKKVMLYVGGLRPR
HVVGAYEDLGMEVVGAGYEFAHGDDYQRTTHYVKDGTLIYDDVTGFEFEKFAEKIRPDLV
GSGVKEKYIFQKMGIPFRQMHSWDYSGPYHGYDGFAIFARDMDMAINSPVWSMTKAPWKK