Protein Info for Dsui_0847 in Dechlorosoma suillum PS

Annotation: 16S rRNA (guanine(527)-N(7))-methyltransferase GidB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 213 PF02527: GidB" amino acids 28 to 208 (181 residues), 176.4 bits, see alignment E=3.8e-56 TIGR00138: 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG" amino acids 30 to 211 (182 residues), 180.1 bits, see alignment E=1.7e-57

Best Hits

Swiss-Prot: 61% identical to RSMG_THIDA: Ribosomal RNA small subunit methyltransferase G (rsmG) from Thiobacillus denitrificans (strain ATCC 25259)

KEGG orthology group: K03501, ribosomal RNA small subunit methyltransferase G [EC: 2.1.1.170] (inferred from 65% identity to app:CAP2UW1_4362)

Predicted SEED Role

"rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.170

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QI93 at UniProt or InterPro

Protein Sequence (213 amino acids)

>Dsui_0847 16S rRNA (guanine(527)-N(7))-methyltransferase GidB (Dechlorosoma suillum PS)
MSGFSQAQQLAQGLDALGIPLDGAAQGKLLAYLALLAKWNKVYALTALRDQDKAVSHHLL
DSLAILPFVDALAPANVLDVGSGGGQPGIPLAIARPSLAVTLLDSNSKKVAFLKQAAMEL
PLPGLSAQCLRVEAWQPEQKFAAITSRAFAEVADFVSLTRHLLAPGGRWLAMKGIYPQEE
LSRLPAGIVVEAVHPLAVPGVEGARHLVVLREE