Protein Info for Dsui_0747 in Dechlorosoma suillum PS
Annotation: glutamate--cysteine ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01919, glutamate--cysteine ligase [EC: 6.3.2.2] (inferred from 79% identity to dar:Daro_0011)Predicted SEED Role
"Glutamate--cysteine ligase (EC 6.3.2.2), divergent, of Alpha- and Beta-proteobacteria type" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle (EC 6.3.2.2)
MetaCyc Pathways
- glutathione biosynthesis (2/2 steps found)
- ophthalmate biosynthesis (2/3 steps found)
- homoglutathione biosynthesis (1/2 steps found)
- γ-glutamyl cycle (3/6 steps found)
- ergothioneine biosynthesis I (bacteria) (3/7 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 6.3.2.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8QH64 at UniProt or InterPro
Protein Sequence (426 amino acids)
>Dsui_0747 glutamate--cysteine ligase (Dechlorosoma suillum PS) MVPHLTTALTGPLLDLEKRFLDDSTRIEQWLRSQWQEHTPPFYGSVDLRNNGFKLAPVDT NLFPGGFNNLNPAFLPLCVHATMSAIEKICPDARNLLLIPENHTRNQFYLMNVAQLATIL RQTGLNVRIGTLLPEITEPTTLELPNGHSLVLEPLVRTQYRLGLKDFDPCAILLNNDLSA GAPAILQNLNEQFLLPPLHAGWAVRRKSHHFAAYDQVATDFAAHLGLDPWRINPYFAVCN QINFHERQGEDCLAAQVDMLLARIREKYKEYGVTETPYVVVKADAGTYGMGVMTVKDASE VTGLNRKQRNKMSVVKEGLEVSEVIIQEGVHTFESINDGVAEPVVYMIDRYVVGGFYRVH SGRAKDENLNAPGMHFEPLAFETCCNQPEHALAPDAPPNRFYAYGVVARLALLAASVELE NTAPAA