Protein Info for Dsui_0742 in Dechlorosoma suillum PS

Annotation: phosphoenolpyruvate-protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 579 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 5 to 572 (568 residues), 542.9 bits, see alignment E=4.2e-167 PF05524: PEP-utilisers_N" amino acids 6 to 130 (125 residues), 102.7 bits, see alignment E=2.4e-33 PF00391: PEP-utilizers" amino acids 165 to 235 (71 residues), 78.6 bits, see alignment E=3.5e-26 PF02896: PEP-utilizers_C" amino acids 262 to 549 (288 residues), 364.6 bits, see alignment E=5.5e-113

Best Hits

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] (inferred from 73% identity to dar:Daro_4084)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QH59 at UniProt or InterPro

Protein Sequence (579 amino acids)

>Dsui_0742 phosphoenolpyruvate-protein phosphotransferase (Dechlorosoma suillum PS)
MSFTIHGLGVSGGIAIGVAQLMSHATLEVAHLTIGPRMADKEVLRFEAAILAVQAELEQL
KLEASKGNAPAELRAFVDLHGMILADPELSEVPKQIIRERRCNAEWAVVQQMERLVAQFD
AIDDAYLRERKADVVQVVERVVKVLMGHPGRSALRARKGGKETTDQIIVAHDLSPADTIG
FKEQRFASFITDVGGATSHTAILARSMAIPSIVGLHRARDLIRDGELLIIDGTRGVVIVN
PDERVLEEYRLRKSELELERSKLKRLKTMKATTLDGVDVLMYANIELPEDVEAARTAGAD
GIGLFRTEFLFLNRGDMPTEDEQFEAYRKVVKGMEGKPVTIRTFDLGADKALDAMDRVKT
NPALGLRAIRLQLAEPKMFRTQLRAILRASKYGKVKILIPMLAHAHEIDATLAAVQAAKQ
SLREQKIGFDEGIEVGGMIEIPAAALAIGMFLRRLDFLSIGTNDLIQYALAIDRSDETVS
QLYDPLHPAVLMLVAHTIYSGEKAGLPVSVCGELAGDPAMTRLLLGMGLRVFSMHPSQIL
EVKQHVLKSEVAEIAPQVRKILRYSEPEKIREQIDKLNA