Protein Info for Dsui_0715 in Dechlorosoma suillum PS

Annotation: 3,4-dihydroxy-2-butanone 4-phosphate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00506: 3,4-dihydroxy-2-butanone-4-phosphate synthase
  • amino acids 9 to 205 (197 residues), 230.8 bits, E=5.9e-73
• PF00926: DHBP_synthase
  • amino acids 14 to 204 (191 residues), 275.5 bits, E=2.1e-86
• PF00925: GTP_cyclohydro2
  • amino acids 219 to 367 (149 residues), 124.4 bits, E=3.6e-40

Best Hits

Swiss-Prot: 58% identical to RIBB_NEIMB: 3,4-dihydroxy-2-butanone 4-phosphate synthase (ribB) from Neisseria meningitidis serogroup B (strain MC58)

KEGG orthology group: K14652, 3,4-dihydroxy 2-butanone 4-phosphate synthase / GTP cyclohydrolase II [EC: 3.5.4.25 4.1.99.12] (inferred from 70% identity to azo:azo0319)

Predicted SEED Role

"3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12)" (EC 4.1.99.12)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) (3/5 steps found)
• toxoflavin biosynthesis (3/7 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.5.4.25

Use Curated BLAST to search for 3.5.4.25 or 4.1.99.12

Sequence Analysis Tools

See G8QH32 at UniProt or InterPro

Protein Sequence (373 amino acids)

>Dsui_0715 3,4-dihydroxy-2-butanone 4-phosphate synthase (Dechlorosoma suillum PS)
MNAATASAISPIQDIIADIRAGKMVILVDEEDRENEGDVIIASDFITPEAINFMVTHCRG
LVCLTITEERSRQLGLTQMARDNKTPYGTAFTVSIEAAEGVTTGISAHDRARTVQAAVAK
NAGPDDIVQPGHIFPITAKPGGVLVRAGHTEAGCDLGALAGLEPSSVICEILKEDGTMAR
LPDLIEFAKLHGLKIGTIADLIHYRSEHETLVEPLRVKTVQTPHGEFQLHAFHDRVADEV
HLALVKGEIKADQETLVRVHEPVSVLDFLDPAGGVHTVSIDQAQQALAKHGSGVIVLLHR
PQSGSDLRAALCPEKAAAPAPKKWDPRIYGIGAQILRQLGVRKMKLLSSPRRMPSMTGFG
LEVTGFVTDIEKP