Protein Info for Dsui_0551 in Dechlorosoma suillum PS

Annotation: nitrogenase molybdenum-iron cofactor biosynthesis protein NifN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 461 signal peptide" amino acids 27 to 28 (2 residues), see Phobius details amino acids 33 to 35 (3 residues), see Phobius details TIGR01285: nitrogenase molybdenum-iron cofactor biosynthesis protein NifN" amino acids 3 to 427 (425 residues), 605 bits, see alignment E=4.3e-186 PF00148: Oxidored_nitro" amino acids 19 to 424 (406 residues), 385.6 bits, see alignment E=1.2e-119

Best Hits

Swiss-Prot: 58% identical to NIFN_BRADU: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN (nifN) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02592, nitrogenase molybdenum-iron protein NifN (inferred from 62% identity to rva:Rvan_1957)

MetaCyc: 44% identical to Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN (Azotobacter vinelandii)
RXN-17026

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifN" in subsystem Nitrogen fixation

MetaCyc Pathways

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QFF1 at UniProt or InterPro

Protein Sequence (461 amino acids)

>Dsui_0551 nitrogenase molybdenum-iron cofactor biosynthesis protein NifN (Dechlorosoma suillum PS)
MAEVKHSRKACAVNPLKMSQPIGAALAFLGMDQALPLLHGSQGCTAFGLVLFVKHFRETI
PLQTTAMNEVTTILGGLENVEQALVNIHKRAAPALVGLISTGITEAKGDDVDGFLQLIRQ
KHPELADMEVVFVSTPDFAGAFQDGWGKAVLRMVETIAKPSNVRRPWQVNILPGCHLTPG
DIEEVKEMVEAFGLTPVVLPDLSGSMDGHIPETFLPTTLGGTRLDELRSMGESAYTLVIG
EQGRAAAAKLKDIAGVPYTVFDRLTGLKPCDDFLALLSRIANTEVPPRFKRQRSQLLDAM
LDGHFHFGKKKVAVAAEPDLLYALTTFLTEMGAEIQVAVTSTESPLLNRVPADEVLIGDL
EDFEQGAVGADLLITHAHGRQAAARLGIPFWRSGLPTFDRLGAAHKVSVGYRGTRNLIFE
VGNVFIENLHEAVPDDFRPTAEPAASCGTGCAHGAAAVACH