Protein Info for Dsui_0519 in Dechlorosoma suillum PS

Updated annotation (from data): Methylmalonyl-CoA mutase (EC 5.4.99.2)
Rationale: Specifically important for utilizing Sodium propionate. Automated validation from mutant phenotype: the predicted function (METHYLMALONYL-COA-MUT-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 37 to 558 (522 residues), 894.1 bits, E=2.4e-273
• PF01642: MM_CoA_mutase
  • amino acids 39 to 551 (513 residues), 796.9 bits, E=7.8e-244
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 590 to 719 (130 residues), 196.2 bits, E=1.8e-62
• PF02310: B12-binding
  • amino acids 593 to 704 (112 residues), 66.6 bits, E=1.9e-22

Best Hits

Swiss-Prot: 66% identical to MUTA_HUMAN: Methylmalonyl-CoA mutase, mitochondrial (MMUT) from Homo sapiens

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 92% identity to dar:Daro_4006)

MetaCyc: 70% identical to methylmalonyl-CoA mutase (Cereibacter sphaeroides)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• propanoyl CoA degradation I (3/3 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• conversion of succinate to propanoate (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• pyruvate fermentation to propanoate I (4/7 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (7/13 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• L-glutamate degradation VIII (to propanoate) (4/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (3/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 5.4.99.2

Use Curated BLAST to search for 5.4.99.2

Sequence Analysis Tools

See G8QFC3 at UniProt or InterPro

Protein Sequence (721 amino acids)

>Dsui_0519 Methylmalonyl-CoA mutase (EC 5.4.99.2) (Dechlorosoma suillum PS)
MSADASKPQLPNSDNLDAWAKAAAKSAPGGDVNALNWITPEGLTVKPLYTKKDVEDLPYA
DTLPGFAPYLRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKALAAGGQGVSVAFDLAT
HRGYDSDNPRVLGDVGKAGVAIDSVEDMKILFDGIPLDKISVSMTMNGAVLPILAGYIVA
AEEQGVSQEQLSGTIQNDILKEFMVRNTYIYPPKPSMKIISDIFGYTAQHMPKFNSISIS
GYHIQEAGANQAIELAFTLADGMEYVRTGIASGLDVDAFAGRLSFFWAVGMNFYLEIAKM
RAGRMLWHRIMSQFNPKSAKSLMLRTHSQTSGWSLTEQDPYNNVVRTTIEAMAAVFGGTQ
SLHTNALDEAIALPTEFSARIARNTQLIIQEETHICNVVDPWAGSYMMEKLTQDMADKAW
SIIQEIEAMGGMTKAVESGWAKMQVETCAADKQARIDSGKDVIVGVNKYKLAKEDQIDIL
DIDNHAVREAQIARLKKIRASRDSAAVQAALDALTQCAESGEGNLLDLSVKAIRLRATVG
EVSDALEKVFGRFRANNQTISGVYGGVVEGQESWESIKADIAKFAEEEGRRPRIMIAKLG
QDGHDRGAKVVATAFADLGFDIDMGPLFQTPEEAARQAVENDVHAIGVSSLAAGHKTLLP
ALVNSLKEQGADDIIVFAGGVIPAQDYDTLYAAGAKAIFGPGTRIEDSAKRVLEEIRKSR
G