Protein Info for Dsui_0517 in Dechlorosoma suillum PS

Updated annotation (from data): Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3)
Rationale: Specifically important for utilizing Sodium propionate. Automated validation from mutant phenotype: the predicted function (PROPIONYL-COA-CARBOXY-RXN) was linked to the condition via a MetaCyc pathway. This annotation was also checked manually.
Original annotation: acetyl-CoA carboxylase, carboxyltransferase component (subunits alpha and beta)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01039: Carboxyl_trans
  • amino acids 27 to 508 (482 residues), 687 bits, E=1.4e-210
• PF03255: ACCA
  • amino acids 305 to 446 (142 residues), 36.4 bits, E=3.5e-13

Best Hits

Swiss-Prot: 74% identical to PCCB_RHOS4: Propionyl-CoA carboxylase beta chain (pccB) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K01966, propionyl-CoA carboxylase beta chain [EC: 6.4.1.3] (inferred from 91% identity to azo:azo0687)

MetaCyc: 74% identical to propionyl-CoA carboxylase beta subunit (Cereibacter sphaeroides)
Propionyl-CoA carboxylase. [EC: 6.4.1.3]

Predicted SEED Role

"Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 6.4.1.3)

MetaCyc Pathways

• superpathway of anaerobic energy metabolism (invertebrates) (14/17 steps found)
• propanoyl CoA degradation I (3/3 steps found)
• 2-oxobutanoate degradation I (3/4 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• superpathway of L-methionine salvage and degradation (9/16 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• mycolate biosynthesis (15/205 steps found)
• superpathway of mycolate biosynthesis (16/239 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 6.4.1.3

Use Curated BLAST to search for 6.4.1.3

Sequence Analysis Tools

See G8QFC1 at UniProt or InterPro

Protein Sequence (510 amino acids)

>Dsui_0517 Propionyl-CoA carboxylase carboxyl transferase subunit (EC 6.4.1.3) (Dechlorosoma suillum PS)
MHDIIHELEKKREAARLGGGQKRIDSQHKKGKLTARERLELLLDPDSFEEWDMFKEHRCT
DFGMAETKNPGDGVVTGYGTINGRLVFVFSQDFTVFGGSLSETHAEKICKVMDHAMKVGA
PVIGLNDSGGARIQEGVASLGGYADVFQRNVMASGVIPQISMIMGPCAGGAVYSPAMTDF
IFMVKDSSYMFVTGPEVVKTVTHEEVTAEELGGAVTHTTKSGVADLAFENDVEALNYLRR
LVNFLPANNREKPPVQKTNDPAERLDFSLDTLVPDNANKPYDMKELIIKMVDDCDFFEIQ
PDYAKNIITGFARMDGHPVGIVANQPLVLAGCLDIKSSIKAARFVRFCDAFNIPVVTLVD
VPGFMPGTSQEYGGIIKHGAKLLYAYAECTVPKVTLITRKAYGGAYDVMSSKHLRGDVNL
AWPSAEIAVMGPKGAVEIIFREEKNDPAKLAEREAEYKAKFANPFVAGARGFIDDVIMPN
ETRKRICRSLAMLRDKKLDNPWRKHGNIPL