Protein Info for Dsui_0500 in Dechlorosoma suillum PS

Annotation: (p)ppGpp synthetase, RelA/SpoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 743 PF13328: HD_4" amino acids 62 to 211 (150 residues), 165.1 bits, see alignment E=3.7e-52 TIGR00691: RelA/SpoT family protein" amino acids 62 to 737 (676 residues), 764.8 bits, see alignment E=4.1e-234 PF01966: HD" amino acids 81 to 180 (100 residues), 39.2 bits, see alignment E=2.6e-13 PF04607: RelA_SpoT" amino acids 271 to 381 (111 residues), 131.3 bits, see alignment E=7e-42 PF02824: TGS" amino acids 424 to 483 (60 residues), 72.3 bits, see alignment 9.6e-24 PF19296: RelA_AH" amino acids 509 to 578 (70 residues), 28.1 bits, see alignment E=5.2e-10 PF28438: RelA_RIS" amino acids 596 to 657 (62 residues), 84.8 bits, see alignment 9.1e-28 PF13291: ACT_4" amino acids 662 to 738 (77 residues), 63.8 bits, see alignment E=6.5e-21

Best Hits

KEGG orthology group: None (inferred from 68% identity to app:CAP2UW1_0930)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QFA4 at UniProt or InterPro

Protein Sequence (743 amino acids)

>Dsui_0500 (p)ppGpp synthetase, RelA/SpoT family (Dechlorosoma suillum PS)
MDTATDPAPAKPSASSAAPFAPAPDPAAPPTPYPFNDDPAYRVFLDSLDYLKPEEIAKIK
EAFAFGEAAHRGQKRLSGEPYITHPLAVAGAIAEWRLDSTAIIAALLHDTMEDTGISKEE
LTERFGKGVADLVDGLSKLDKIEFSSYQEAQAENFRKMLLAMAKDLRVILIKLTDRLHNM
QTLGCMRPDKRRRIALETLEIYAPIANRLGLNTVYRELQDLSFKHTHPMRYQVLLKAVMA
ARGNRREVLSKILDGVQSKMRDSGIEAQVFGREKSLYSIYRKMVEKRLSFSQVLDIYGFR
VVVKDVPSCYLGLGALHALYKPLPGKFKDYIAIPKANGYQSLHTTLIGPYGMPVEVQLRT
EEMHHMAQEGVASHWLYKDTEKSAAELQYQTHRWLQSLLELQSTAGDSAEFFEHVKIDLF
PDEVYVFSPKGKIFSLPKGATPVDFAYAVHTDVGNRCVAAKINYELMPLRSELNSGDQVE
IVTAAHANPNPAWLSYVKTGRARSKIRHFLKTRQHEESAALGERLLNQELFGLGITPSEL
PDASWEAVLKEGGSKSVKEVYTDIGLGRRLAAVVARRLLAHEAALPNAEPAPHTSVVIRG
TEGMAIQLAHCCRPIPGDPIIGSIKKGQGLVVHTHDCAVIRKSRSAEPQRWIDVEWEPEP
GKLFDVDIHVAARNARGVLAKVATEIAESGSNIEKVSMAPDPGFYTTLNFTVQVANRAHL
ARVLRAVRLIPEVVRITRERQEE