Protein Info for Dsui_0323 in Dechlorosoma suillum PS

Annotation: 3-hydroxyacyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01782, 3-hydroxyacyl-CoA dehydrogenase / enoyl-CoA hydratase / 3-hydroxybutyryl-CoA epimerase [EC: 1.1.1.35 4.2.1.17 5.1.2.3] (inferred from 76% identity to dar:Daro_1547)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.1.2.3, EC 5.3.3.8)

MetaCyc Pathways

oleate β-oxidation (28/35 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
fatty acid salvage (5/6 steps found)
pyruvate fermentation to butanol II (engineered) (5/6 steps found)
1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
adipate degradation (4/5 steps found)
fatty acid β-oxidation IV (unsaturated, even number) (4/5 steps found)
fatty acid β-oxidation III (unsaturated, odd number) (1/1 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
L-isoleucine degradation I (4/6 steps found)
oleate β-oxidation (thioesterase-dependent, yeast) (1/2 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
adipate biosynthesis (3/5 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (18/26 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (4/7 steps found)
2-methyl-branched fatty acid β-oxidation (9/14 steps found)
oleate β-oxidation (reductase-dependent, yeast) (1/3 steps found)
methyl ketone biosynthesis (engineered) (3/6 steps found)
propanoate fermentation to 2-methylbutanoate (3/6 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (5/9 steps found)
valproate β-oxidation (5/9 steps found)
glycerol degradation to butanol (10/16 steps found)
4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) (2/5 steps found)
pyruvate fermentation to butanol I (4/8 steps found)
oleate β-oxidation (isomerase-dependent, yeast) (1/4 steps found)
pyruvate fermentation to butanoate (3/7 steps found)
6-gingerol analog biosynthesis (engineered) (2/6 steps found)
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
L-valine degradation I (3/8 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (5/11 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
benzoyl-CoA degradation I (aerobic) (2/7 steps found)
3-phenylpropanoate degradation (4/10 steps found)
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) (4/10 steps found)
L-glutamate degradation V (via hydroxyglutarate) (4/10 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
phenylacetate degradation I (aerobic) (3/9 steps found)
2-methylpropene degradation (2/8 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (5/13 steps found)
gallate degradation III (anaerobic) (3/11 steps found)
superpathway of phenylethylamine degradation (3/11 steps found)
methyl tert-butyl ether degradation (2/10 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
L-glutamate degradation VII (to butanoate) (3/12 steps found)
10-cis-heptadecenoyl-CoA degradation (yeast) (2/12 steps found)
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) (2/12 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
L-tryptophan degradation III (eukaryotic) (3/15 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
toluene degradation VI (anaerobic) (3/18 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
androstenedione degradation I (aerobic) (6/25 steps found)
platensimycin biosynthesis (6/26 steps found)
Spodoptera littoralis pheromone biosynthesis (3/22 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
superpathway of testosterone and androsterone degradation (6/28 steps found)
androstenedione degradation II (anaerobic) (4/27 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17, 5.3.3.8

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.1.2.3 or 5.3.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QNE8 at UniProt or InterPro

Protein Sequence (645 amino acids)

>Dsui_0323 3-hydroxyacyl-CoA dehydrogenase (Dechlorosoma suillum PS)
MNMHTQTSLTQQHWHLTREADGLAWLSLDKAGESANSLSKAVMAELSGVLDELDRQPPKA
LIIRSAKSAGFIAGADISEFDQLDSPEAAKAMVARGWELFNRLAAVPYPTLALVRGHCLG
GGLELALACRTLLVVDEPGTKLGLPEVMLGIFPGWGGMLRLPRRVGPAVALDLMLAGKTV
DAKKAKRLGLADECVPPRVMESAARQLALNPPSRKPLPLLQRLFLGPLRGVVAGQARKQV
AKRARPEHYPAPYAIIDLWAKYDGNALAAPEITDRIVRSATARNLVRVFHLQERLKAFGK
DSAFVAKRVHVVGAGVMGGDIAAWCAGRGLTVTLQDQNVERIAPAIKRAHAGFARRIRDK
LQLRAVMDRLIPDPEGHGAAHADVVIEAIFENLEAKHSLLKGLEARMKPDAVLATNTSSL
KLEDIRTVLQRPERLVGIHFFNPVAKMPLVEVVTAAGGDAEVTRQACAFVRGIDKLPLPV
QSAPGFLVNAVLGPYLLEAMRCVDEGIAPETVDEAALAFGMPMGPIELADTVGLDIAMAA
GKALAGNGTEPPKCLVQRFERGDLGRKSGKGFYAYPAGKIQKGAAGAVPAGLAERLVQPL
LQRTQQLVDAGVVADAELADAGVIFGTGFAPFTGGPLNYARSRHA