Protein Info for Dsui_0087 in Dechlorosoma suillum PS
Annotation: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 47% identical to MANC9_ECOLX: Mannose-1-phosphate guanylyltransferase (manC) from Escherichia coli
KEGG orthology group: K00971, mannose-1-phosphate guanylyltransferase [EC: 2.7.7.22] K01809, mannose-6-phosphate isomerase [EC: 5.3.1.8] (inferred from 65% identity to pna:Pnap_3192)Predicted SEED Role
"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)
MetaCyc Pathways
- colanic acid building blocks biosynthesis (10/11 steps found)
- D-mannose degradation II (2/2 steps found)
- GDP-mannose biosynthesis (3/4 steps found)
- mannitol degradation II (3/4 steps found)
- β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation (2/3 steps found)
- D-mannose degradation I (1/2 steps found)
- 1,5-anhydrofructose degradation (3/5 steps found)
- mannitol biosynthesis (1/3 steps found)
- superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis (8/14 steps found)
- β-(1,4)-mannan degradation (2/7 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 5.3.1.8
Use Curated BLAST to search for 2.7.7.22 or 5.3.1.8
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See G8QLI2 at UniProt or InterPro
Protein Sequence (478 amino acids)
>Dsui_0087 mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (Dechlorosoma suillum PS) MKNPTITAVILCGGSGTRLWPLSRKSFPKQFVPLLGDKSLLQLTFERTSGLGERVLAVAA EDHRFMVTDDMLAARVAGAVILEPAARNTAAAMALAALQAIQDGLDLLLFCPADHHVPDV EGFLRTIRQGVPAAQAGALVTFGVVPSFPSTAYGYIQRGDSRADGSFHAGRFIEKPDLAR AQELLLAGDNLWNAGIFLCRAVILLEALEAHAPDILLTCRQAMADAQVEVSPSGHAFVRP EARSFGTCRSQSIDYAVMEQHDNVAVVPFEGQWSDVGSWNAVADLQVPDGDGNRVAGQGM VHHSSNTFIHAPHRPVVALGTDNLLIIDTPDAVLVAHRDKAEQVKDVVTRLEQAQCTQAV THRKVARPWGWYDSIDRGERFQVKRIGVKPGASLSLQKHHHRAEHWVVVKGTAEVTCGEN TFLLSENQSTYIPIGEVHRLRNPGVVELEIVEIQSGSYLGEDDIVRLHDTYGRAPQQQ