Protein Info for Dsui_0017 in Dechlorosoma suillum PS

Annotation: 2-haloalkanoic acid dehalogenase, type II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 TIGR01428: haloacid dehalogenase, type II" amino acids 7 to 203 (197 residues), 227.1 bits, see alignment E=2e-71 PF00702: Hydrolase" amino acids 7 to 188 (182 residues), 64.1 bits, see alignment E=2.3e-21 TIGR01493: HAD hydrolase, family IA, variant 2" amino acids 9 to 188 (180 residues), 97.7 bits, see alignment E=8.4e-32 PF13419: HAD_2" amino acids 97 to 196 (100 residues), 38.9 bits, see alignment E=1e-13

Best Hits

Swiss-Prot: 46% identical to HAD_PSEUY: (S)-2-haloacid dehalogenase from Pseudomonas sp. (strain YL)

KEGG orthology group: K01560, 2-haloacid dehalogenase [EC: 3.8.1.2] (inferred from 66% identity to eba:ebA812)

Predicted SEED Role

"Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase" (EC 3.1.3.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.-

Use Curated BLAST to search for 3.1.3.- or 3.8.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See G8QKQ4 at UniProt or InterPro

Protein Sequence (229 amino acids)

>Dsui_0017 2-haloalkanoic acid dehalogenase, type II (Dechlorosoma suillum PS)
MTAPCPIKAIAFDAYGTLFDVYSVGALAEQLFPGKGVALAALWRDKQIEYSRIRSMSGRY
KPFWEVTQDALIYAGAAIGVAMGETERGRLMSQYACLSAFPENLPALKELKTLGLPLAIL
SNGTRAMLDIAVKSSGMGGIFDHILSVEMVQKFKTDPEVYQLGPDAFDLPAREILFVSAN
SWDACAATWFGYTTFWINRTGQPPEQLNVSPTASGHRLSDVVAFVRENQ