Protein Info for CSW01_19235 in Vibrio cholerae E7946 ATCC 55056

Annotation: glycogen debranching enzyme GlgX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 656 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details transmembrane" amino acids 478 to 497 (20 residues), see Phobius details TIGR02100: glycogen debranching enzyme GlgX" amino acids 7 to 636 (630 residues), 674.8 bits, see alignment E=6e-207 PF02922: CBM_48" amino acids 10 to 87 (78 residues), 39.7 bits, see alignment E=5.2e-14 PF00128: Alpha-amylase" amino acids 199 to 249 (51 residues), 22.3 bits, see alignment 7.4e-09

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to vcj:VCD_000312)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (656 amino acids)

>CSW01_19235 glycogen debranching enzyme GlgX (Vibrio cholerae E7946 ATCC 55056)
MKMNLSTPFPLGATLDAEGCNFAIYAPANRDILLALFHADGSYETHQLEHEYAGVKHTHI
SGIHAGQKYGYLIQLNDELHYISDPYARALEGPLHYAPPFDSHKSFDLPKCVVTDTHFDW
QNVAKPRIARDEMVLFETHVKGLTQLNPDVEKALRGKYLGLVSQPMLDFYRQQNINTLQL
LPIAACMHEPHLLESGKVNYWGYNPYVFMAPDPRYASKDAVNELKTTIRELHRNGIQVIL
DVVYNHTAEGGTNGPVFNLKALDPNYYLHHGDHYANYTGCGNTVDLSNQAALNLVMDTLR
CWVTEYQIDGFRFDLAATLGRRGDEFSKEAAFFKAVAQDPVLREVKLIAEPWDIGPNGYQ
VGNFPFGWNETNDKLRDITRSFWRGDLGFLKEFATRLMGSRDLYSAANWPYKLTVNYITY
HDGFTLQDLVSYKHKHNEANGEQNRDGHGDNRSDNYGFEGDTDSIVIRATRERQKRNFMA
SLLFAFGIPHILTADVLSHTQKGNNNAYCQDNDISWLNWENNETKQDFREWLAGMVAARQ
TYMVPFIRAFSGENRNNNRIAWRRVDGKPMEMDDWNRLSSVALHIGIGSDGPEMLYLINQ
TNAPARFVLPKDRQQDWRLICDTNMRHAQHGHAEGEVLQLPVSMSILHYQPKKKSA