Protein Info for CSW01_19050 in Vibrio cholerae E7946 ATCC 55056

Annotation: phosphoenolpyruvate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01418: phosphoenolpyruvate synthase
  • amino acids 19 to 800 (782 residues), 1221.5 bits, E=0
• PF01326: PPDK_N
  • amino acids 30 to 359 (330 residues), 422 bits, E=2.7e-130
• PF00391: PEP-utilizers
  • amino acids 397 to 467 (71 residues), 99.2 bits, E=1.2e-32
• PF02896: PEP-utilizers_C
  • amino acids 494 to 793 (300 residues), 201.7 bits, E=2.3e-63

Best Hits

Swiss-Prot: 72% identical to PPSA_PSEAB: Phosphoenolpyruvate synthase (ppsA) from Pseudomonas aeruginosa (strain UCBPP-PA14)

KEGG orthology group: K01007, pyruvate, water dikinase [EC: 2.7.9.2] (inferred from 72% identity to aha:AHA_2691)

MetaCyc: 70% identical to phosphoenolpyruvate synthetase (Escherichia coli K-12 substr. MG1655)
Pyruvate, water dikinase. [EC: 2.7.9.2]

Predicted SEED Role

"Phosphoenolpyruvate synthase (EC 2.7.9.2)" in subsystem Glycolysis and Gluconeogenesis or Glycolysis and Gluconeogenesis, including Archaeal enzymes or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 2.7.9.2)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of N-acetylneuraminate degradation (20/22 steps found)
• superpathway of glycolysis and the Entner-Doudoroff pathway (16/17 steps found)
• gluconeogenesis I (13/13 steps found)
• hexitol fermentation to lactate, formate, ethanol and acetate (17/19 steps found)
• glycolysis I (from glucose 6-phosphate) (12/13 steps found)
• glycolysis II (from fructose 6-phosphate) (10/11 steps found)
• superpathway of anaerobic sucrose degradation (15/19 steps found)
• superpathway of hexitol degradation (bacteria) (14/18 steps found)
• glycolysis V (Pyrococcus) (7/10 steps found)
• gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (24/56 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.9.2

Protein Sequence (803 amino acids)

>CSW01_19050 phosphoenolpyruvate synthase (Vibrio cholerae E7946 ATCC 55056)
MPTLQAAMFLEKDMQKNTLWFDSLSMQDVDKVGGKNASLGEMVSNLANAGVSVPNGFATT
AYAFNQFLDFEGLDERIHQLLDELDVDDVDALRKTGATIRQWILQAPFPAELEQEIRTNY
NQLIDGNSELSVAVRSSATAEDLPDASFAGQQETFLNVKGIDAVLEATKHVYASLFNDRA
ISYRVHQGFDHRGIALSAGIQRMVRSDKAASGVMFTLDTESGFDKVVFITAAWGLGEMVV
QGAVNPDEFYVHKPLLEAGYPAIVKKTFGSKLSKMIYANSQVIGKQVEVVDTSAHERNQF
SLTDAEIQELAKQALIIEKHYQRPMDIEWAKDGIDGKLYIVQARPETVCSQSEQNVIERY
ELSHKASVLLEGRAIGQRIGSGTVRLVDSLDQMSLVQQGDVLVTDMTDPDWEPVMKKAAA
IVTNRGGRTCHAAIIARELGIPAIVGCGDATRRLSDGAQVTVSCAEGETGYVYAGQLDFA
VRRSSVDELPMLPTKVMMNVGNPDRAFDFAQIPNEGVGLARLEFIINKMIGIHPKALLNF
DTQSAELQTQILDRIRGYRDPIDFYVSKLTEGIATIAAAFWPKRVIVRMSDFKSNEYSNL
LGGSEFEPHEENPMLGFRGASRYISPVFEDCFELETQAIKRVRHEMGLKNVEVMIPFVRT
TGEAEAVIDLLAKFGLRRGEQGLKVIMMCELPSNAVLADEFLKFFDGFSIGSNDMTQLTL
GLDRDSGDVAHLFDERNPAVKIMLKMAIDAATRAGKYVGICGQGPSDHDDLAQWLMEQGI
SSVSLNPDTVIDTWLKLGAVSKC