Protein Info for CSW01_18690 in Vibrio cholerae E7946 ATCC 55056

Annotation: heme anaerobic degradation radical SAM methyltransferase ChuW/HutW

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 455 TIGR04107: putative heme utilization radical SAM enzyme HutW" amino acids 24 to 443 (420 residues), 607.4 bits, see alignment E=6.4e-187 PF04055: Radical_SAM" amino acids 67 to 239 (173 residues), 63 bits, see alignment E=2e-21

Best Hits

KEGG orthology group: K02495, oxygen-independent coproporphyrinogen III oxidase [EC: 1.3.99.22] (inferred from 100% identity to vch:VCA0909)

Predicted SEED Role

"Radical SAM family protein HutW, similar to coproporphyrinogen III oxidase, oxygen-independent, associated with heme uptake" in subsystem Heme and Siroheme Biosynthesis

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.22

Use Curated BLAST to search for 1.3.99.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (455 amino acids)

>CSW01_18690 heme anaerobic degradation radical SAM methyltransferase ChuW/HutW (Vibrio cholerae E7946 ATCC 55056)
MIMLILDNFDESILGANTPDPLRFAFQNKHSAHAGGIAMPVPSHEQANVWQHITQQVSQR
QQVRCLYIHVPFCRVRCTFCNFFQNAASRQLVDAYFAALLEEIKQKAALPWTQTGVFHAV
YIGGGTPTELSPEQIRQLGTAIRESFPLTPDCEITLEGRIHRFSDEMFENALEGGFNRFS
FGVQSFNTQVRRRAKRLDDREVVMERIASLAATQQAPIVIDLLYGLPYQTAQVFEQDLQD
FMQTGAQGIDLYQLVVGGSAPMLNLVEKGKLPPPATTPDKASLYQIGVEFMAKHHLRPLS
VNHWTRDNRERSLYNSLAKTYAEVLPIGCGAGGNMGGYSLMQHRQLDTYLDAMKNGQPLV
AMMARQHEYDPLFAALKAGFDAGVIAKQRLPKFYHHQTFDWLMPLFLRWQQIGLVEVEQD
YLTLTTAGRFWSVSLAQACIQVLIHSYKYQQQRIA