Protein Info for CSW01_18430 in Vibrio cholerae E7946 ATCC 55056

Annotation: alpha-amylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 690 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details PF00128: Alpha-amylase" amino acids 243 to 385 (143 residues), 115 bits, see alignment E=2.8e-37 amino acids 444 to 612 (169 residues), 48.3 bits, see alignment E=5.5e-17

Best Hits

KEGG orthology group: K01176, alpha-amylase [EC: 3.2.1.1] (inferred from 100% identity to vcj:VCD_000470)

Predicted SEED Role

"Periplasmic alpha-amylase (EC 3.2.1.1)" in subsystem Maltose and Maltodextrin Utilization (EC 3.2.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.1

Use Curated BLAST to search for 3.2.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (690 amino acids)

>CSW01_18430 alpha-amylase (Vibrio cholerae E7946 ATCC 55056)
MKLNALTLSLLSALALPALASPASTANLTVATTTNSRDFPLQADEPLVIPLTKGDYTLTI
SGIGGDCPPVPEQEIKFNTPIALNCHVPTQLPLSIRFTGDYAFQWQAQNNTLTFVRQTTK
AAKTEFHRPIPNVSCEVYQGGEVTLDLASSFADGTELQDAMTGQVVTIEQGKVRLTPSAN
SGGLVLLEPKQTAKAEPKQPFTYRNANIYFVMVDRFYNADPSNDGSYGRHKDGQEEIGTF
HGGDLKGVIAKLDHIQSLGTDAIWLSPIVEQVHGFVGGGEKGSFPFYAYHGYWTRDFTKI
DANFGKDEDLQTLVREAHRRGIKILMDAVINHAGYATLADLQQDAVQVVNAPMLPERWND
WKPSADENWHSFHQAIDYQSKNWQQWWGPDWVRAGLPGYPAPGSSDITMNLAGLPDFRTE
SPQAVTPPQWLLNNPGTRVVSKPNYTVADYLIEWQSDWVRRFGIDGFRIDTVKHVEGEVW
QRLKQRATESLAAWRKDNNQSGEPFWMMGEVWGHGAYRSPYFDDGFDALINFDIQKRMDN
GAACLSQMAMVYRDYAQTLAKYPDFNPVSYMSSHDTELFFGRFKSLDMQRNAANALLLTP
GAIQVYYGDEVAREAGPYADDFHQGTRSDMPWEWNAERQALLKHWQTLGQFRQRHPAIGT
GEHREIAQSNAYVFTRTLGEDKVVVAFVGR