Protein Info for CSW01_18185 in Vibrio cholerae E7946 ATCC 55056

Annotation: DEAD/DEAH box family ATP-dependent RNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 663 PF00270: DEAD" amino acids 50 to 216 (167 residues), 168.8 bits, see alignment E=1.9e-53 PF04851: ResIII" amino acids 64 to 210 (147 residues), 27.3 bits, see alignment E=6.4e-10 PF00271: Helicase_C" amino acids 252 to 360 (109 residues), 104.9 bits, see alignment E=5.9e-34 PF03880: DbpA" amino acids 504 to 571 (68 residues), 64.4 bits, see alignment E=1.6e-21

Best Hits

Swiss-Prot: 46% identical to DEAD_BUCAP: ATP-dependent RNA helicase DeaD (deaD) from Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)

KEGG orthology group: K05592, ATP-dependent RNA helicase DeaD [EC: 3.6.4.13] (inferred from 100% identity to vch:VCA0804)

MetaCyc: 46% identical to ATP-dependent RNA helicase DeaD (Escherichia coli K-12 substr. MG1655)
5.6.2.e [EC: 5.6.2.e]

Predicted SEED Role

"DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13)" (EC 3.6.4.13)

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13 or 5.6.2.e

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (663 amino acids)

>CSW01_18185 DEAD/DEAH box family ATP-dependent RNA helicase (Vibrio cholerae E7946 ATCC 55056)
MVGNGKARGTLVISSFSGIPMQDTAIQFSDLALNSAILSALTEMGFVSPTPIQAAAIPVL
LEGRDALGKAQTGTGKTAAFSLPLLNKLNLSQYKPQAIVMAPTRELAIQVAAEIKNLGQN
IKGLKVLEIYGGASILDQMRALKSGAHIVVGTPGRVKDLITRDRLHLDECHTFILDEADE
MLKMGFVDDVTWIMEQAPESAQRVLFSATMPPMVKEIVERFLRNPECVDVAGSNQTVAKV
EQQYWVVKGVEKDEAMARLLETEETDASIVFVRTRQDTERLADWLCARGFKAAALHGDIP
QSLRERTVDHIKQGVIDILVATDVVARGLDVPRITHVYNYDIPFDVESYIHRIGRTGRAG
RKGKAILLVRTNQIRMLRTIERVTRSSMEEIQLPHRDKVAESRLTKLGQELAADKEFSSL
ERFADLVEKLQASLEIDATTLAAILLKRQQGKRPLFYVGADPMIEAMEREKSRRRERRDD
RRDGDRPARREFGGRDQENHDWDTYQLQVGRDQGVQVKDIVGALANELGLTKGSIGAIKL
AQGHTFVQLPKAMSNDVSSKLRKLRIRQKEVGAVVCDFDDFRESRGGARREGGPRREGGR
RPEGNREGGFRGGREGGREGGRGRGGEGRPFREGERRFERNRSGESSGSYRGERGHSRRR
EEA