Protein Info for CSW01_18020 in Vibrio cholerae E7946 ATCC 55056

Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 PF04851: ResIII" amino acids 28 to 196 (169 residues), 41.6 bits, see alignment E=1.9e-14 PF00270: DEAD" amino acids 30 to 202 (173 residues), 177.3 bits, see alignment E=3.2e-56 PF00271: Helicase_C" amino acids 238 to 346 (109 residues), 103.3 bits, see alignment E=1.4e-33

Best Hits

Swiss-Prot: 41% identical to RHLB_PSEPK: ATP-dependent RNA helicase RhlB (rhlB) from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)

KEGG orthology group: K11927, ATP-dependent RNA helicase RhlE [EC: 3.6.4.13] (inferred from 100% identity to vcm:VCM66_A0727)

Predicted SEED Role

"ATP-dependent RNA helicase VCA0768" in subsystem ATP-dependent RNA helicases, bacterial

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.13

Use Curated BLAST to search for 3.6.4.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (422 amino acids)

>CSW01_18020 DEAD/DEAH box helicase (Vibrio cholerae E7946 ATCC 55056)
MSELSITFSQLGLDSRLLNTLSELGIANPTPIQQQAIPHVLQGKDVLAGAQTGTGKTAAF
GLPLIQRFIEQPWQREANSKEIRALVLVPTRELAQQVLDSLQAYAKGTELKIVAVYGGTS
MKVQLNHLRGGVDILIATPGRLLDHAHVKSLFLGKVEVLVLDEADRMLDMGFMPDLQRVL
RRLSPLRQTLFFSATFDSKIKAVAYRIMRDPIEVQVTPSNSTAETVQQMVYPVDKKRKRE
LLSYLIGSKNWQQVLVFTKTRQGSDALAEELKLDGIKAVSINGDKSQGARQKALDDFKAG
KVRALIATDVAARGLDIAQLEQVVNYDMPFKAEDYVHRIGRTGRAGLAGLAVSLLSHDEQ
PQLEAIERLLNTRLPQEWLKGFEPSPVVHEENSPRRSAGRSSEKRKLKAQLKIHAQRGKK
AR