Protein Info for CSW01_17580 in Vibrio cholerae E7946 ATCC 55056

Annotation: periplasmic nitrate reductase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 829 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details TIGR01706: periplasmic nitrate reductase, large subunit" amino acids 2 to 828 (827 residues), 1679 bits, see alignment E=0 PF04879: Molybdop_Fe4S4" amino acids 42 to 94 (53 residues), 70.1 bits, see alignment 1.9e-23 PF00384: Molybdopterin" amino acids 98 to 570 (473 residues), 226.1 bits, see alignment E=1e-70 PF01568: Molydop_binding" amino acids 714 to 822 (109 residues), 90.5 bits, see alignment E=1.1e-29

Best Hits

Swiss-Prot: 87% identical to NAPA_ALIF1: Periplasmic nitrate reductase (napA) from Aliivibrio fischeri (strain ATCC 700601 / ES114)

KEGG orthology group: K02567, periplasmic nitrate reductase NapA [EC: 1.7.99.4] (inferred from 76% identity to avr:B565_1207)

MetaCyc: 87% identical to nitrate reductase large subunit (Aliivibrio fischeri)
Nitrate reductase (cytochrome). [EC: 1.9.6.1]

Predicted SEED Role

"Periplasmic nitrate reductase precursor (EC 1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.7.99.4 or 1.9.6.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (829 amino acids)

>CSW01_17580 periplasmic nitrate reductase subunit alpha (Vibrio cholerae E7946 ATCC 55056)
MKMTRRAFVKANAAASAAAVAGITLPASATNLIASSDQTAIHWDKAPCRFCGTGCSVLVG
TQDGRVVATQGDPEAPVNKGLNCIKGYFLSKIMYGQDRLKTPLLRMKDGQYHKDGEFTPV
SWDTAFDVMAEKWKASLKTKGPTSVGMFGSGQWTVMEGYAAVKLMKAGFRSNNIDPNARH
CMASAVVGFMRTFGIDEPMGCYDDFEHADAFVLWGSNMAEMHPVLWTRITDRRLSHPHVK
VNVLSTYYHRSFELADHGYIFHPQSDLAIANFIANYIIQNDAVNWDFVNKHTHFKQAVTD
IGYGLRDDHPLQKKAKNANSGDVSDISFEEYKKSVAPYTVEKASEISGVSPDKLITLAKQ
YADPNTKVMSLWTMGMNQHTRGVWMQSLVYNLHLLTGKIATPGNSPFSLTGQPSACGTAR
EVGTFAHRLPADMVVANPKHRAIAEKVWKLPEGTIPEKPGFHAVQQDRMLKDGVLNCYWV
QCNNNMQAGPNINEERLPGYRNPENFIVVSDAYPTVTAQAADLVLPTAMWVEKEGAYGNA
ERRTQVWYQQVKTVGESHSDSWQVIEFSKRFKVEDVWPEELLAKAPQYRGKTLYDVLFKN
GQVDKFPLSEARELNDDAHHFGFYIQKGLFEEYAEFGRGHGHDLAPYDVYHQVRGLRWPV
VDGKETKWRFKEGSDPYAKAGSGWDFYGKPDGKAWIISSPYEAPPEMPNEEYDLWLCTGR
VLEHWHTGTMTRRVPELYKAVPDALCFMHHEDAQARGLRRGDEVLISNSRGEVRVRVETR
GRNKPPKGLVFVPFFDARILVNKLILDATDPLSKQTDFKKCPVKITKVA