Protein Info for CSW01_17535 in Vibrio cholerae E7946 ATCC 55056
Annotation: L-serine ammonia-lyase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 56% identical to SDHL_ECOLI: L-serine dehydratase 1 (sdaA) from Escherichia coli (strain K12)
KEGG orthology group: K01752, L-serine dehydratase [EC: 4.3.1.17] (inferred from 100% identity to vco:VC0395_0608)MetaCyc: 56% identical to L-serine deaminase I (Escherichia coli K-12 substr. MG1655)
L-serine ammonia-lyase. [EC: 4.3.1.17]
Predicted SEED Role
"L-serine dehydratase, beta subunit (EC 4.3.1.17) / L-serine dehydratase, alpha subunit (EC 4.3.1.17)" in subsystem Glycine and Serine Utilization or Pyruvate Alanine Serine Interconversions (EC 4.3.1.17)
MetaCyc Pathways
- superpathway of L-lysine, L-threonine and L-methionine biosynthesis II (13/15 steps found)
- D-serine degradation (3/3 steps found)
- L-serine degradation (3/3 steps found)
- L-tryptophan degradation II (via pyruvate) (3/3 steps found)
- glycine degradation (3/3 steps found)
- L-methionine biosynthesis II (5/6 steps found)
- L-cysteine degradation II (2/3 steps found)
- glycine betaine degradation III (4/7 steps found)
- felinine and 3-methyl-3-sulfanylbutan-1-ol biosynthesis (2/5 steps found)
- L-mimosine degradation (4/8 steps found)
- glycine betaine degradation I (4/8 steps found)
- glutathione-mediated detoxification I (3/8 steps found)
- purine nucleobases degradation II (anaerobic) (12/24 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 4.3.1.17
Use Curated BLAST to search for 4.3.1.17
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (476 amino acids)
>CSW01_17535 L-serine ammonia-lyase (Vibrio cholerae E7946 ATCC 55056) MRFVPSYLIGFITLYLKGKVMLSIFDIYKIGVGPSSSHTNGPMIAGYRFVQQLLPQLNKV ARIQVDLYGSLSLTGKGHHTDRATLLGLMGNQPATIKIGDANQTLREALQTGKLRLNGQH DIAFDYHQDLLFHQDNLPLHENGMTLSAFDTQGQQIDFETYYSVGGGFVATAEQLQNGTA TADVTVTYPFTSADEMLHQAEKHGLSLGGMILRNELAFQEMAVIDAKADQIWRVMSQCME RGFATEGILEGGLNVTRRAPSLLKKLEANAAIENDPMEVMDWINLFAFAVSEENAAGGQV VTSPTNGAAGVIPAVLMYYHRFIKALDTKQLKDFLAVSGAIGILYKTNASISGAEVGCQG EVGVSSSMAAAGLTALRGGSNEQICMAAEIAMEHSLGMTCDPIGGLVQVPCIERNAMGAV KAINASRMALKRTSKCLISLDKVIETMYQTGKDMNKKYRETSLGGLALIHLAPPCE