Protein Info for CSW01_14055 in Vibrio cholerae E7946 ATCC 55056

Annotation: tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 631 transmembrane" amino acids 599 to 618 (20 residues), see Phobius details TIGR00136: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA" amino acids 7 to 622 (616 residues), 969.3 bits, see alignment E=3.7e-296 PF12831: FAD_oxidored" amino acids 8 to 151 (144 residues), 40.1 bits, see alignment E=7.7e-14 PF00890: FAD_binding_2" amino acids 8 to 37 (30 residues), 22.2 bits, see alignment (E = 1.9e-08) PF01134: GIDA" amino acids 8 to 399 (392 residues), 585 bits, see alignment E=2e-179 PF21680: GIDA_C_1st" amino acids 460 to 556 (97 residues), 92.1 bits, see alignment E=7.9e-30 PF13932: SAM_GIDA_C" amino acids 561 to 616 (56 residues), 81.8 bits, see alignment 6.1e-27

Best Hits

Swiss-Prot: 100% identical to MNMG_VIBC3: tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG (mnmG) from Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 / O395)

KEGG orthology group: K03495, glucose inhibited division protein A (inferred from 100% identity to vco:VC0395_A2517)

MetaCyc: 78% identical to 5-carboxymethylaminomethyluridine-tRNA synthase subunit MnmG (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (631 amino acids)

>CSW01_14055 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG (Vibrio cholerae E7946 ATCC 55056)
MLYHETFDVIVVGGGHAGTEAALAAARTGQRTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
KEVDAMGGLMAQAIDHAGIQFRTLNASKGPAVRATRAQADRALYKAYVRNVLENTPNLTL
FQQAVDDVIVEHDHIRGVVTQMGLKFHAKAVVLTVGTFLGGKIHIGLENYAGGRAGDPPS
IALAHRLRELPFRVDRLKTGTPPRIDANSVDFSVLEAQHGDNPTPVFSFMGKREHHPRQI
PCYITHTNERTHDVIRANLDRSPMYAGIIEGIGPRYCPSIEDKVMRFADKDSHQIFIEPE
GLTTTELYPNGISTSLPFDVQVQIVRSMKGFENAHIVRPGYAIEYDFFDPRDLKQTYETK
YIHGLFFAGQINGTTGYEEAAAQGLMAGLNASLYSQDKEGWSPRRDQAYMGVLIDDLSTM
GTKEPYRMFTSRAEYRLLLREDNADLRLTEKARELGLVDDARWARFNQKIDNMAKERQRL
QETWMNPNSVGVEQLNTLLKTPMSREASGEDLLRRPEMTYELLTTLPAFAPALEDAEAAE
QVEIQVKYDGYIQRQQDEIEKSLRHEHTKLPAELDYKQVKGLSNEVVLKLNAAKPETIGI
ASRISGITPAAISILLVHLKKHGMLKKGEAA