Protein Info for CSW01_13990 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01173: UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase
  • amino acids 4 to 452 (449 residues), 697 bits, E=5.5e-214
• PF00483: NTP_transferase
  • amino acids 5 to 206 (202 residues), 47.1 bits, E=6e-16
• PF01128: IspD
  • amino acids 5 to 128 (124 residues), 32.8 bits, E=1.6e-11
• PF12804: NTP_transf_3
  • amino acids 6 to 126 (121 residues), 80.6 bits, E=3.7e-26
• PF00132: Hexapep
  • amino acids 392 to 426 (35 residues), 40 bits, 5.1e-14
• PF14602: Hexapep_2
  • amino acids 392 to 426 (35 residues), 33.5 bits, 6.8e-12

Best Hits

Swiss-Prot: 100% identical to GLMU_VIBCH: Bifunctional protein GlmU (glmU) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K04042, bifunctional UDP-N-acetylglucosamine pyrophosphorylase / Glucosamine-1-phosphate N-acetyltransferase [EC: 2.3.1.157 2.7.7.23] (inferred from 100% identity to vco:VC0395_A2530)

MetaCyc: 68% identical to fused N-acetylglucosamine-1-phosphate uridyltransferase and glucosamine-1-phosphate acetyltransferase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylglucosamine diphosphorylase. [EC: 2.7.7.23]; Glucosamine-1-phosphate N-acetyltransferase. [EC: 2.7.7.23, 2.3.1.157]

Predicted SEED Role

"N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157)" in subsystem Peptidoglycan Biosynthesis or Sialic Acid Metabolism or UDP-N-acetylmuramate from Fructose-6-phosphate Biosynthesis (EC 2.3.1.157, EC 2.7.7.23)

MetaCyc Pathways

• peptidoglycan recycling I (13/14 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis I (5/5 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (5/7 steps found)
• UDP-N-acetyl-D-glucosamine biosynthesis II (4/6 steps found)
• chitin biosynthesis (5/9 steps found)
• O-antigen building blocks biosynthesis (E. coli) (6/11 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (8/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (9/33 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Nucleotide sugars metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.157 or 2.7.7.23

Protein Sequence (453 amino acids)

>CSW01_13990 bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase (Vibrio cholerae E7946 ATCC 55056)
MKFSTVILAAGKGTRMHSNMPKVLHTLAGKPMVKHVIDTCNNLGAQNIHLVYGHGGDQMQ
QALANENVNWVLQAQQLGTGHAVDQASPHFQDDEKILVLYGDVPLISEDTIESLLEAQPT
DGIALLTVVLEDPTGYGRIVRKRGPVVAIVEQKDASEEQKLIKEVNTGVLVATGRDLKRW
LAGLNNNNAQGEYYLTDVIAAAHDEGRAVEAVHPSHSIEVEGVNDRIQLARLERAFQARQ
AKKLLEQGVMLRDPARFDLRGTLQCGSDVEIDVNVIIEGNVSIGNNVVIGAGSILKDCEI
DDNTVIRPYSVIEGATVGENCTVGPFTRLRPGAELRDDAHVGNFVEMKNARLGEGSKANH
LTYLGDAEIGKGVNVGAGVITCNYDGANKHKTVIGDDVFVGSDCQLVAPVTIGNGATIGA
GTTLTKNVAEGELVITRAPERKIAGWQRPAKKK