Protein Info for CSW01_13680 in Vibrio cholerae E7946 ATCC 55056

Annotation: DNA helicase RecG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 692 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF17191: RecG_wedge" amino acids 15 to 170 (156 residues), 70.8 bits, see alignment E=3.8e-23 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 28 to 663 (636 residues), 784.3 bits, see alignment E=5.3e-240 PF01336: tRNA_anti-codon" amino acids 63 to 133 (71 residues), 42.9 bits, see alignment E=1.3e-14 PF04851: ResIII" amino acids 267 to 429 (163 residues), 47.1 bits, see alignment E=9e-16 PF00270: DEAD" amino acids 269 to 435 (167 residues), 88.3 bits, see alignment E=1.7e-28 PF00271: Helicase_C" amino acids 478 to 586 (109 residues), 70.8 bits, see alignment E=3.9e-23 PF19833: RecG_dom3_C" amino acids 616 to 675 (60 residues), 53 bits, see alignment 1e-17

Best Hits

Swiss-Prot: 70% identical to RECG_ECO57: ATP-dependent DNA helicase RecG (recG) from Escherichia coli O157:H7

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 100% identity to vcm:VCM66_2631)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (692 amino acids)

>CSW01_13680 DNA helicase RecG (Vibrio cholerae E7946 ATCC 55056)
MSQLLSAVPLCELSGVGAKVAEKLEKVGLHTVQDLLFHLPLRYEDRTRVYPIVQLHHGLW
AAVQGKVMAVDTLFGKRKMLTVKISDGNGTLTLRFFNFTAAMKNNFAEGKFVHAYGEIKR
GNQGLEIIHPDYKFFTPAQTPDVEPNLTPVYPTTEGLRQLTLRNLTDQALVLLEKSAVQE
LLPSGLYDQQMTLAQALKIIHRPSADIDLRLFEQGRHPAQIRLIMEELLAQNLSMLAIRS
QGQQDVALPLAPVHQLKQQLLAQLPFSPTKAQQRVVAEIEADLAKPHPMMRLVQGDVGSG
KTLVAALAAVRAIEHGYQVALMAPTELLAEQHALNFAQWLEPMGIQVGWLAGKLKGKARE
TELARIASGEVKMVVGTHALFQEQVSFDHLALVIIDEQHRFGVHQRLELREKGAKQGAYP
HQLIMTATPIPRTLAMTAYADLETSVIDELPPGRTPIQTVAIPDTKRDEIVERIRHACLN
EGKQAYWVCTLIDESEVLEAQAAAETAEELQRKLPEVKIGLVHGRMKPAEKQAVMQAFKN
NELHLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGTVASHCVLLFHAP
LSKTAQKRLGVLRESNDGFVIAQRDLEIRGPGELLGTKQTGLADFKIADLVRDQQLVPQV
QRIARHIHERYPQNAQAIIDRWLGERDIYAKA