Protein Info for CSW01_13675 in Vibrio cholerae E7946 ATCC 55056

Annotation: bifunctional GTP diphosphokinase/guanosine-3',5'-bis(diphosphate) 3'-diphosphatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 705 TIGR00691: RelA/SpoT family protein" amino acids 27 to 702 (676 residues), 899.1 bits, see alignment E=9.5e-275 PF13328: HD_4" amino acids 28 to 175 (148 residues), 182.7 bits, see alignment E=1.2e-57 PF01966: HD" amino acids 45 to 144 (100 residues), 43.4 bits, see alignment E=1.2e-14 PF04607: RelA_SpoT" amino acids 235 to 345 (111 residues), 139.3 bits, see alignment E=1.9e-44 PF02824: TGS" amino acids 389 to 447 (59 residues), 71.4 bits, see alignment 1.5e-23 PF19296: RelA_AH_RIS" amino acids 461 to 542 (82 residues), 40.3 bits, see alignment E=1.1e-13 PF13291: ACT_4" amino acids 626 to 702 (77 residues), 56.5 bits, see alignment E=1.1e-18

Best Hits

Swiss-Prot: 100% identical to SPOT_VIBCH: Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 100% identity to vch:VC2710)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (705 amino acids)

>CSW01_13675 bifunctional GTP diphosphokinase/guanosine-3',5'-bis(diphosphate) 3'-diphosphatase (Vibrio cholerae E7946 ATCC 55056)
MYLFDSLKDVAQEYLTEPQIEALRQSYVVARDAHEGQTRSSGEPYIIHPVAVARILAEMR
LDLETLQAALLHDVIEDCDVTKEDLDAHFGSSVAELVDGVSKLDKLKFRDRKEAQAENFR
KMVLAMVQDIRVILIKLADRTHNMRTLGALRPDKKRRIARETLEIYAPLAHRLGIHNIKT
ELEELGFEALYPNRYRVLKEVVKAARGNRKEMIQRIHSEIEGRLQEVGLPARVVGREKNL
FSIYNKMKTKEQRFHTIMDIYAFRIVVDTADTCYRVLGQVHSLYKPRPARMKDYIAVPKA
NGYQSLHTSMVGPHGVPVEVQIRTEDMDQMADKGVAAHWSYKANSERGGTTAQIKAQRWM
QSLLELQQSAGNSFEFIENVKSDLFPDEIYVFTPKGRIVELPMGATAVDFAYAVHTDIGN
TCVGARVDRTPYPLSQSLKSGQTVEIISAPGARPNAAWLNYVVTSRARTKIRQVLKTMRR
EDSITLGRRLLNHALGEHSVNEIAPENISKVLSDLKIASMDDLLAAIGLGELMSIVIARR
LLGNADELTEPSKSGGNKNKLPIRGAEGILLTFANCCHPIPDDHIIAHVSPGRGLVVHRE
TCPNVRGYQKEPDKYMAVEWTKDYDQEFITELKVDMHNRQGALAELTNVISKTGSNIHGL
STEERDGRLYTVTVLLTTKDRVHLAGIMRKIRTMPHALKVRRRKN