Protein Info for CSW01_13620 in Vibrio cholerae E7946 ATCC 55056

Annotation: aspartate ammonia-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00839: aspartate ammonia-lyase
  • amino acids 16 to 482 (467 residues), 842.3 bits, E=5.7e-258
• PF00206: Lyase_1
  • amino acids 23 to 355 (333 residues), 333.2 bits, E=1.9e-103
• PF10415: FumaraseC_C
  • amino acids 421 to 474 (54 residues), 67.9 bits, 8.1e-23

Best Hits

Swiss-Prot: 81% identical to ASPA_HAEIN: Aspartate ammonia-lyase (aspA) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K01744, aspartate ammonia-lyase [EC: 4.3.1.1] (inferred from 100% identity to vcj:VCD_001670)

MetaCyc: 78% identical to aspartate ammonia-lyase (Escherichia coli K-12 substr. MG1655)
Aspartate ammonia-lyase. [EC: 4.3.1.1]

Predicted SEED Role

"Aspartate ammonia-lyase (EC 4.3.1.1)" in subsystem Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 4.3.1.1)

MetaCyc Pathways

• superpathway of L-aspartate and L-asparagine biosynthesis (4/4 steps found)
• L-aspartate degradation II (aerobic) (3/3 steps found)
• L-aspartate degradation III (anaerobic) (3/3 steps found)
• L-glutamate degradation II (2/2 steps found)
• L-canavanine degradation II (1/6 steps found)

KEGG Metabolic Maps

• Alanine and aspartate metabolism
• Nitrogen metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.3.1.1

Protein Sequence (483 amino acids)

>CSW01_13620 aspartate ammonia-lyase (Vibrio cholerae E7946 ATCC 55056)
MATLSEAPKTATPATRVEEDLLGQRHVPADAYYGIHTLRAVENFNISNVTISDVPEFVRG
MIMTKKAATLANKELGVIPKDVANYILQACDLILETGKCMDQFPSDVFQGGAGTSVNMNT
NEVIANVALELMGKQKGQYEFINPNDHVNKSQSTNCAYPTGFRIAVYNSILKVIDAIEYL
KGSFELKAAEYKNILKMGRTQLQDAVPMTVGQEFHAWAVTLNEEIRALQYTSKLLLEVNL
GATAIGTGLNAAPGYQELAVKYLAQVTGLETVPAEDLIEATSDCGAYVMTHGALKRLAMK
LSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVIPEVVNQVCFKVFGNDNTI
TFAAEAGQLQLNVMEPVIAQAMFESISLLTNACVNLRDKCIDGITVNKEICENFVFNSIG
IVTYLNPYIGHHEGDIVGKICAETGKSVREVVLERGLLTEEQLDDIFSVENLMHPQYKAK
RYE