Protein Info for CSW01_13580 in Vibrio cholerae E7946 ATCC 55056

Annotation: cation-efflux pump FieF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 302 signal peptide" amino acids 7 to 11 (5 residues), see Phobius details transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 41 to 61 (21 residues), see Phobius details amino acids 82 to 102 (21 residues), see Phobius details amino acids 118 to 139 (22 residues), see Phobius details amino acids 159 to 176 (18 residues), see Phobius details amino acids 182 to 199 (18 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 13 to 288 (276 residues), 228.5 bits, see alignment E=5.1e-72 PF01545: Cation_efflux" amino acids 15 to 207 (193 residues), 156 bits, see alignment E=1.1e-49 PF16916: ZT_dimer" amino acids 211 to 287 (77 residues), 92.6 bits, see alignment E=1.3e-30

Best Hits

Swiss-Prot: 64% identical to FIEF_PECAS: Cation-efflux pump FieF (fieF) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K13283, ferrous-iron efflux pump FieF (inferred from 100% identity to vcj:VCD_001678)

MetaCyc: 59% identical to Zn2+/Fe2+/Cd2+ exporter (Escherichia coli K-12 substr. MG1655)
RXN0-6; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (302 amino acids)

>CSW01_13580 cation-efflux pump FieF (Vibrio cholerae E7946 ATCC 55056)
MMKKEYARLVTMAAWTATAVASLLLVVKVAAWWVTGSVSLLASLIDSVLDIAASVVNLIV
LRFALQPADHEHAFGHGKAESLAALAQAMFISGSACFLILNGVDRFFRPHELNAPELGIY
VSAIAILVTFGLVLFQRYVVRKTGSQAIAADSLHYQSDLLMNAAIMVALGLSWWGLGQAD
AIFAIGIGLYILFSAFKMVHDAVQTLLDRKLPDEELEQIHAICLSVAGVIGMHQLRTRMS
GPVRFIQLHLELDDNISLLEAHQISDRVEDKLREHFVGADVLIHQDPHSVVLEAEQQQKS
LQ